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Catalog Number Size Price
LS-C60557-1 1 mg (1 mg/ml) $376 
Human IgG Antibody - Dot Blot of Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed at 1:1,000 for 1 HR at RT.

Polyclonal Goat Anti‑Human IgG Secondary Antibody (AP, IHC, WB) LS‑C60557

Polyclonal Goat Anti‑Human IgG Secondary Antibody (AP, IHC, WB) LS‑C60557

Antibody:
Human IgG Goat anti-Human Polyclonal (AP) Antibody
Application:
IHC, WB, ELISA, DB
Reactivity:
Human
Format:
Alkaline Phosphatase, Unmodified
Price
Catalog Number
$376
LS-C60557-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Human IgG Goat anti-Human Polyclonal (AP) Antibody
Application:
IHC, WB, ELISA, DB
Reactivity:
Human
Format:
Alkaline Phosphatase, Unmodified

Specifications

Description
Human IgG antibody LS-C60557 is an AP-conjugated goat polyclonal antibody. Validated for DB, ELISA, IHC and WB.
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Human IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins..
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2500)
  • ELISA (1:2000 - 1:10000)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024