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Anti-HTT / Huntingtin Antibody (phospho-Ser421) IHC-plus™ LS-B36

Ordering

Wt. Vol. Conc. Price
50 µg - 0.8 mg/ml $395

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HTT / Huntingtin Antibody LS-B36 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-HTT / Huntingtin antibody IHC of human brain cerebellum.
Western Blot
Anti-Huntingtin pS421 Antibody - Western Blot.
Western Blot
Anti-Huntingtin pS421 Antibody - Western Blot.
 

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Rabbit Polyclonal to Human HTT / Huntingtin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human HTT / Huntingtin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (10 - 20 µg/ml), Western blot (1:500 - 1:3000), ELISA (1:10000 - 1:40000)

Specificity and Use

HTT / Huntingtin antibody was raised against synthetic peptide from human HTT / Huntingtin.
pSer421
Amino acids 416 to 424 of Human huntingtin protein phosphorylated at Ser421
Immunohistochemistry: LS-B36 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B36 was determined to be 10-20 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HTT / Huntingtin

P42858 NM_002111 NP_002102.4

HTT Antibody, Huntingtin Antibody, Huntington disease protein Antibody, IT15 Antibody, HD Antibody, HD protein Antibody

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological.

Immunohistochemistry

Immunohistochemistry
Anti-HTT / Huntingtin antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B36 concentration 20 ug/ml.

Western Blot

Western Blot
Anti-Huntingtin pS421 Antibody - Western Blot. Western blot analysis after AKT and phosphatase treatment is shown using Affinity Purified anti-Huntingtin pS421 antibody. In A) untreated lysates from N2A and 293A cells were stained directly using anti-Huntingtin pS421 antibody. Full length staining of Huntingtin is noted, albeit at low levels of expression, as well as a strongly staining band at 200 kD that may represent staining of truncated protein. In B) staining is shown after immunoprecipitation using a monoclonal antibody (Mab2166) followed by AKT treatment (to phosphorylate), along with untreated, and phosphatase (PP) treated (dephosphorylate) immunoprecipitated. Full length phosphorylated huntingtin is clearly detected in these immunopurified samples (except dephosphorylated). In C) lysates are treated directly with AKT or PP to alter the phosphorylation status of Htt. Personal communication, Simon Warby, CMMT, Vancouver, BC.

Western Blot

Western Blot
Anti-Huntingtin pS421 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Huntingtin pS421 antibody to detect endogenous protein present in an unstimulated human PC-3 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~190 kD corresponding to truncated human Huntingtin protein. The blot was incubated with a 1:1000 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication,.
 

Requested From: United States
Date Requested: 10/21/2014

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