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Catalog Number Size Price
LS-B2441-50 50 µg (1 mg/ml) $645 
HPSE / Heparanase Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
HPSE / Heparanase Antibody - Negative Staining in Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
HPSE / Heparanase Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
HPSE / Heparanase Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
HPSE / Heparanase Antibody - Western blot analysis of Anti-HPSE antibody (LS-B2441, 1:2000 dilution). Lane 1: Purified recombinant human Heparanase (Control). Lane 2: Extract from 6.5x10e3 CHO cells transfected with human Heparanase. Lane 3: Extract from 8x10e4 CHO cells transfected with mouse Heparanase. Lane 4: Extract from 5-10e5 non-transfected CHO dhfr- cells. Antibody produced band at ~65 kDa (precursor) & ~50 kDa and 8 kDa (subunits)
HPSE / Heparanase Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
HPSE / Heparanase Antibody - Negative Staining in Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
HPSE / Heparanase Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
HPSE / Heparanase Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
HPSE / Heparanase Antibody - Western blot analysis of Anti-HPSE antibody (LS-B2441, 1:2000 dilution). Lane 1: Purified recombinant human Heparanase (Control). Lane 2: Extract from 6.5x10e3 CHO cells transfected with human Heparanase. Lane 3: Extract from 8x10e4 CHO cells transfected with mouse Heparanase. Lane 4: Extract from 5-10e5 non-transfected CHO dhfr- cells. Antibody produced band at ~65 kDa (precursor) & ~50 kDa and 8 kDa (subunits)
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IHC‑plus™ Polyclonal Rabbit anti‑Human HPSE / Heparanase Antibody (IHC, WB) LS‑B2441

IHC‑plus™ Polyclonal Rabbit anti‑Human HPSE / Heparanase Antibody (IHC, WB) LS‑B2441

Note: This antibody replaces LS-C24191, LS-C67434
Antibody:
HPSE / Heparanase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$645
LS-B2441-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HPSE / Heparanase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Heparanase antibody LS-B2441 is an unconjugated rabbit polyclonal antibody to Heparanase (HPSE) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human HPSE / Heparanase
Synonyms
HPSE | Endo-glucoronidase | Heparanase-1 | Heparanase | HPA | HSE1 | HEP | HPA1 | HPSE1 | HPR1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A heterodimeric recombinant protein consisting of the 50 kDa and 8 kDa subunits.
Specificity
Recognizes the 65kD precursor as well as the 50kD and 8kD subunits of human or mouse Heparanase.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2441 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2441 was determined to be 5 ug/ml. Western Blot: 1:1000.
Presentation
PBS, pH 7.2, 0.01% Thimerosal
Storage
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HPSE / Heparanase
Q9Y251 NM_006665 NP_006656.2

Validation

HPSE / Heparanase Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

HPSE / Heparanase Antibody - Negative Staining in Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Negative Staining in Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Negative Staining in Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

HPSE / Heparanase Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

HPSE / Heparanase Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ HPSE / Heparanase Antibody for IHC, WB/Western LS-B2441 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Inhibition of Heparanase in Pediatric Brain Tumor Cells Attenuates their Proliferation, Invasive Capacity, and In Vivo Tumor Growth. Spyrou A, Kundu S, Haseeb L, Yu D, Olofsson T, Dredge K, Hammond E, Barash U, Vlodavsky I, Forsberg-Nilsson K. Molecular cancer therapeutics. 2017 August;16:1705-1716.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/20/2024