Human (tested or 100% immunogen sequence identity)
Rat, Bovine, Dog, Pig, Rabbit, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000), ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)
Specificity and Use
HPRT1 / HPRT antibody was raised against recombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (SwissProt P00492). Percent identity by BLAST analysis: Human (100%); Rabbit (98%); Pig (97%); Dog, Rat (96%); Chicken, Bovine (94%); Zebrafish (93%); Xenopus (92%).
Human HPRT1 / HPRT
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.