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| Catalog Number | Size | Price |
|---|---|---|
| LS-C121234-100 | 100 µl | $836 |
Polyclonal Rabbit anti‑Mouse HIF1A / HIF1 Alpha Antibody (Biotin, aa530‑825, IHC, WB) LS‑C121234
Polyclonal Rabbit anti‑Mouse HIF1A / HIF1 Alpha Antibody (Biotin, aa530‑825, IHC, WB) LS‑C121234
Antibody:
HIF1A / HIF1 Alpha Rabbit anti-Mouse Polyclonal (aa530-825) (Biotin) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Rat, Primate
Format:
Biotin, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HIF1A / HIF1 Alpha Rabbit anti-Mouse Polyclonal (aa530-825) (Biotin) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Rat, Primate
Format:
Biotin, Unmodified
Specifications
Description
HIF1 Alpha antibody LS-C121234 is a biotin-conjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa530-825) from mouse. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Mouse HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Mouse, Human, Rat, Primate
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A fusion protein including aa 530-825 of the mouse HIF-1 alpha protein.
Epitope
aa530-825
Specificity
Recognizes mouse HIF-1 alpha. Species cross-reactivity: rat, human, primate. Antibody has been tested in Western Blot, recognizing a band in hypoxic samples at ~115kD. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples.
Applications
- IHC
- IHC - Paraffin (5 - 10 µg/ml)
- Western blot (1:500 - 1:2000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for Western Blot and Immunohistochemistry. Not recommended for Immunoprecipitation. Western Blot: 1:500-1:2000. Immunohistochemistry (regular/paraffin): 5-10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, No preservatives added
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIF1A / HIF1 Alpha
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Species:
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Applications:
IHC, IHC - Paraffin
Species:
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Applications:
IHC, IHC - Paraffin, Western blot
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Species:
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Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
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IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
