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Catalog Number Size Price
LS-C154779-100 100 µg (0.5 mg/ml) $503 
HDAC1 Antibody - Goat Anti-Histone Deacetylase 1 Antibody Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
HDAC1 Antibody - Goat Anti-Histone Deacetylase 1 Antibody (0.1µg/ml) staining of Human Duodenum (A) and Lymph Node (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
HDAC1 Antibody - HDAC1 antibody (0.5 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HDAC1 Antibody - ChIP of 2ug Goat Anti-Histone Deacetylase 1 Antibody with 1ug MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.
HDAC1 Antibody - Goat Anti-Histone Deacetylase 1 Antibody Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
HDAC1 Antibody - Goat Anti-Histone Deacetylase 1 Antibody (0.1µg/ml) staining of Human Duodenum (A) and Lymph Node (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
HDAC1 Antibody - HDAC1 antibody (0.5 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HDAC1 Antibody - ChIP of 2ug Goat Anti-Histone Deacetylase 1 Antibody with 1ug MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.
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Polyclonal Goat anti‑Human HDAC1 Antibody (aa469‑480, IF, WB) LS‑C154779

Polyclonal Goat anti‑Human HDAC1 Antibody (aa469‑480, IF, WB) LS‑C154779

Antibody:
HDAC1 Goat anti-Human Polyclonal (aa469-480) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Guinea pig, Hamster, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C154779-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HDAC1 Goat anti-Human Polyclonal (aa469-480) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Guinea pig, Hamster, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
HDAC1 antibody LS-C154779 is an unconjugated goat polyclonal antibody to HDAC1 (aa469-480) from human. It is reactive with human, mouse, rat and other species. Validated for IF, Peptide-ELISA and WB.
Target
Human HDAC1
Synonyms
HDAC1 | Histone deacetylase 1 | GON-10 | HD1 | RPD3 | RPD3L1
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Guinea pig, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KPEAKGVKEEVK, from the C-Terminus of protein sequence according to NP_004955.2.
Epitope
aa469-480
Specificity
Human HDAC1.
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.1 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 55kD band observed in Human Lymph Node and Thymus lysates and approx 70kD band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1kD according to NP_004955.2). The observed 70kD band represents the sumoylated protein according to David et al, J Biol Chem. 2002 Jun 28;277(26):23658-63; PMID: 11960997. Recommended concentration: 0.5-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HDAC1
Q13547 NM_004964 NP_004955.2

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Requested From: United States
Date Requested: 4/23/2024