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Anti-H2AX Antibody (Ser139) IHC-plus™ LS-B1474

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

H2AX Antibody LS-B1474 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-H2AX antibody IHC of human testis.
Immunohistochemistry
Anti-H2AX antibody IHC of human colon.
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X(Ab-139) antibody.
Western Blot
Western blot of extracts from HepG2 and 293 cells using Histone H2A.X(Ab-139) antibody.

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Rabbit Polyclonal (IgG) to Human H2AX
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human H2AX
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (10 µg/ml), Immunofluorescence, Western blot (1:500 - 1:1000)

Specificity and Use

H2AX antibody was raised against synthetic peptide from human H2AFX / H2AX.
Ser139
Amino acids surrounding Ser 139 of human H2AFX
Immunohistochemistry: LS-B1474 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1474 was determined to be 10 ug/ml.

Packaging

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About H2AX

P16104 NM_002105 NP_002096.1

H2AFX Antibody, H2AX Antibody, Histone H2A.x Antibody, H2A.X Antibody, H2A histone family, member X Antibody, H2AX histone Antibody


Immunohistochemistry

Immunohistochemistry
Anti-H2AX antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1474 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-H2AX antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1474 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X(Ab-139) antibody.

Western Blot

Western Blot
Western blot of extracts from HepG2 and 293 cells using Histone H2A.X(Ab-139) antibody.

Requested From: United States
Date Requested: 9/3/2014

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