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Anti-GSK3B / GSK3 Beta Antibody (phospho-Ser9) IHC-plus™ LS-B55

See our new GSK3B / GSK3 Beta ELISA Kits
Human Phospho-GSK3B / GSK3 Beta Cell-Based ELISA Kit - LS-F1521
Human GSK3B / GSK3 Beta Cell-Based ELISA Kit - LS-F2031
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

GSK3B / GSK3 Beta Antibody LS-B55 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-GSK3B / GSK3 Beta antibody IHC of human ovary.
Western Blot
Anti-GSK3B pS9 Antibody - Western Blot.

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Rabbit Polyclonal to Human GSK3B / GSK3 Beta
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA, Gel shift
Unconjugated

Details

Human GSK3B / GSK3 Beta
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Immunofluorescence (1:200 - 1:1000), Western blot (1:500 - 1:3000), Immunoprecipitation, ELISA (1:10000 - 1:50000), Gel shift

Specificity and Use

GSK3B / GSK3 Beta antibody was raised against synthetic peptide from human GSK3B / GSK3 Beta.
pSer9
The aa 4-12 of human GSK3 beta.
Immunohistochemistry: LS-B55 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B55 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GSK3B / GSK3 Beta

P49841 NM_002093 NP_002084.2

GSK3B Antibody, GSK-3 beta Antibody, GSK3 beta Antibody, GSK3beta Antibody, GSK3beta isoform Antibody, TPKI Antibody, GSK 3beta Antibody, Tau protein kinase I Antibody, ZGSK-3beta Antibody

Glycogen Synthase Kinase 3 beta is a GSK-3 multisubstrate serine/threonine kinase that is associated with the canonical Wnt signaling pathway, which is involved in mammalian brain regionalization. Glycogen Synthase Kinase 3 beta has been implicated in Alzheimer's Disease, when improper phosphorylation of the microtubule-associated protein Tau results in the accumulation of neurofibrillary tangles. Two alternatively spliced isoforms have been observed.

Immunohistochemistry

Immunohistochemistry
Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B55 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-GSK3B pS9 Antibody - Western Blot. Western blot of Rabbit-anti-GSK3B pS9 antibody at a 1:1000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kD. Cells were serum starved for 24 h prior to extraction. Key: Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20, lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20. Molecular weight markers confirm a MW of ~49 kD. Use a 1:2000 dilution of HRP Goat-a-Rabbit IgG (LS-C60865) for detection.

Requested From: United States
Date Requested: 10/1/2014

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