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Anti-GLI2 Antibody (aa46-60) IHC-plus™ LS-B2808

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

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GLI2 Antibody LS-B2808 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-GLI2 antibody IHC of human skin.
Western Blot
Anti-Gli-2 antibody shows detection of Gli-2 protein in rat testis (lane 1) and human HEK293...

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Rabbit Polyclonal to Human GLI2
Human, Chimpanzee
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human GLI2
Rabbit
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (1:500 - 1:2000), ELISA (1:2000 - 1:12000)

Specificity and Use

GLI2 antibody was raised against corresponds to amino acids 46-60 of human Gli-2 (isoform a).
aa46-60
Amino acids 46-60 of human Gli-2 (isoform a).
Immunohistochemistry: LS-B2808 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2808 was determined to be 5 ug/ml. Western Blot: Multiple splice variants have been reported for this protein. Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GLI2

P10070 NM_005270 NP_005261.2

GLI2 Antibody, GLI-Kruppel family member GLI2 Antibody, HPE9 Antibody, Gli-2 Antibody, Oncogene GLI2 Antibody, Tax helper protein 2 Antibody, THP Antibody, Zinc finger protein GLI2 Antibody, Tax helper protein Antibody, THP2 Antibody, GLI family zinc finger 2 Antibody, Tax helper protein 1 Antibody, THP1 Antibody

Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is also thought to play a role during embryogenesis.

Immunohistochemistry

Immunohistochemistry
Anti-GLI2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2808 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-Gli-2 antibody shows detection of Gli-2 protein in rat testis (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead).

Requested From: United States
Date Requested: 10/25/2014

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