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Anti-GJB2 / CX26 / Connexin 26 Antibody (Cytoplasmic Domain) IHC-plus™ LS-B5511

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

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GJB2 / CX26 / Connexin 26 Antibody LS-B5511 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-GJB2 antibody IHC of rat intestine.

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Mouse Monoclonal (IgG1,k) to Rat GJB2 / CX26 / Connexin 26
Rat, Human, Mouse
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated

Details

Rat GJB2 / CX26 / Connexin 26
Mouse
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Immunoaffinity purified

Applications

IHC - Paraffin (10 µg/ml), IHC - Frozen, Western blot (1 µg/ml), ELISA (0.1 - 1 µg/ml)

Specificity and Use

GJB2 / CX26 / Connexin 26 antibody was raised against synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26. (4,5).
Cytoplasmic Domain
Reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5kD. Although the polyclonal anti-Cx26 antibody (feline. No. 71-0500) raised against the same sequence as this monoclonal anti-Cx26 antibody cross-reacts with Cx30, this monoclonal anti-Cx26 appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions. Rat, mouse and human. Reactivity of this antibody on Western blots has been confirmed by using extracts from mouse liver, mouse brain and rat brain.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Immunohistochemistry (Frozen): 1-2 ug/ml. Western Blot: 1 ug/ml. Reactivity confirmed using extracts from mouse liver, mouse brain and rat brain.

Packaging

PBS, pH 7.4, 0.1 % sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12months.
For research use only.

About GJB2 / CX26 / Connexin 26

P29033 NM_004004 NP_003995.2

GJB2 Antibody, Connexin 26 Antibody, Connexin-26 Antibody, DFNB1A Antibody, DFNA3 Antibody, DFNB1 Antibody, Gap junction protein beta 2 Antibody, KID Antibody, HID Antibody, PPK Antibody, NSRD1 Antibody, CX26 Antibody, DFNA3A Antibody


Immunohistochemistry

Immunohistochemistry
Anti-GJB2 antibody IHC of rat intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5511 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 8/29/2014

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