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Anti-G6PC / Glucose-6-phosphatase Antibody (aa72-121) IHC-plus™ LS-B4009

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

G6PC / Glucose-6-phosphatase Antibody LS-B4009 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-G6PC antibody IHC of human kidney.
Immunohistochemistry
Anti-G6PC antibody IHC of human liver.
Immunohistochemistry
Anti-G6PC antibody IHC of human breast.
Western Blot
Western blot of Fetal Lung Lysate.

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Rabbit Polyclonal (IgG) to Human G6PC / Glucose-6-phosphatase
Human, Gibbon, Monkey, Mouse, Dog, Hamster, Pig
IHC - Paraffin, Western blot
Unconjugated

Details

Human G6PC / Glucose-6-phosphatase
Rabbit
Human, Gibbon, Monkey, Mouse, Dog, Hamster, Pig (tested or 100% immunogen sequence identity)
Rat, Bovine, Guinea pig, Horse, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (1 µg/ml)

Specificity and Use

G6PC / Glucose-6-phosphatase antibody was raised against synthetic peptide located between aa72-121 of human G6PC (P35575, NP_000142). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Marmoset, Hamster, Elephant, Dog, Pig (100%); Rat, Panda, Cat, Bovine, Rabbit, Horse, Guinea pig (92%); Monkey, Mouse, Opossum (85%).
LS-E16247 - Lyophilized - 100 µg - $145.00
aa72-121
Human G6PC
Immunohistochemistry: LS-B4009 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4009 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About G6PC / Glucose-6-phosphatase

P35575 NM_000151 NP_000142.1

G6PC Antibody, G6Pase-alpha Antibody, G6PC1 Antibody, G-6-Pase Antibody, GSD1 Antibody, Glucose-6-phosphatase alpha Antibody, G6Pase Antibody, G6PT Antibody, Glucose-6-phosphatase Antibody, GSD1a Antibody

Glucose-6-phosphatase is an integral membrane protein of the endoplasmic reticulum that catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate. It is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Defects in the enzyme cause glycogen storage disease type I (von Gierke disease).

Immunohistochemistry

Immunohistochemistry
Anti-G6PC antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4009 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-G6PC antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4009 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-G6PC antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4009 concentration 5 ug/ml.

Western Blot

Western Blot
Western blot of Fetal Lung Lysate.

Requested From: United States
Date Requested: 10/21/2014

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