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Anti-FGFR1 Antibody (C-Terminus) IHC-plus™ LS-B3031

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

FGFR1 Antibody LS-B3031 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-FGFR1 antibody IHC of human kidney.
Immunohistochemistry
Anti-FGFR1 antibody IHC of human small intestine.
Western Blot
Antibody (0.3 ug/ml) staining of human breast lysate (35 ug protein in RIPA buffer).
 

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Goat Polyclonal to Human FGFR1
Human, Gorilla, Orangutan, Gibbon, Monkey, Bat, Bovine, Dog, Horse
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human FGFR1
Goat
Human, Gorilla, Orangutan, Gibbon, Monkey, Bat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Rat, Hamster, Pig (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (4 µg/ml), Western blot (0.3 - 1 µg/ml), ELISA (1:32000)

Specificity and Use

FGFR1 antibody was raised against synthetic peptide CLPRHPAQLANGGLKR from the C-terminus of human FGFR1 (NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Platypus (100%); Rat, Hamster, Pig, Opossum (94%); Mouse, Rabbit (88%).
LS-E25828 - Lyophilized - 100 µg - $145.00
C-Terminus
Human FGFR1. This antibody is expected to recognise five isoforms (as represented by NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1).
Immunohistochemistry: LS-B3031 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3031 was determined to be 4 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About FGFR1

P11362 NM_023110 CAA47375.1

FGFR1 Antibody, BFGF receptor Antibody, BFGF-R Antibody, BFGF-R-1 Antibody, CD331 Antibody, Cek1 Antibody, CD331 antigen Antibody, FGFR1/PLAG1 fusion Antibody, FLT2 Antibody, Fgf-flg Antibody, FGFBR Antibody, FGFR-1 Antibody, Fms-like tyrosine kinase 2 Antibody, HBGFR Antibody, HH2 Antibody, Hydroxyaryl-protein kinase Antibody, FGF receptor 1 Antibody, Fms-related tyrosine kinase 2 Antibody, Proto-oncogene c-Fgr Antibody, BFGFR Antibody, CEK Antibody, FLT-2 Antibody, HBGF receptor Antibody, KAL2 Antibody, OGD Antibody

The fibroblast growth factor receptor 1 belongs to the FGF Receptor subfamily. The full-length protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. Ligand binding results in the activation of a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. Various isoforms of FGFR1 have been identified that differ in structure and specificity.

Immunohistochemistry

Immunohistochemistry
Anti-FGFR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3031 concentration 4 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-FGFR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3031 concentration 4 ug/ml.

Western Blot

Western Blot
Antibody (0.3 ug/ml) staining of human breast lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 9/19/2014

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