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Anti-FANCA Antibody (aa995-1009) IHC-plus™ LS-B70

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

FANCA Antibody LS-B70 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-FANCA antibody IHC of human brain, cerebellum.
Western Blot
Anti-FANCA Antibody - Western Blot.
Western Blot
Anti-FANCA Antibody - Western Blot.
 

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Rabbit Polyclonal to Human FANCA
Human, Chimpanzee
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human FANCA
Rabbit
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (2.5 µg/ml), Western blot (1:500 - 1:3000), ELISA (1:15000 - 1:60000)

Specificity and Use

FANCA antibody was raised against synthetic peptide from human FANCA.
aa995-1009
Amino acids 995-1009 of human FANCA protein.
Immunohistochemistry: LS-B70 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B70 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (1)

1
Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair. Smogorzewska A, Matsuoka S, Vinciguerra P, McDonald ER, Hurov KE, Luo J, Ballif BA, Gygi SP, Hofmann K, D'Andrea AD, Elledge SJ. Cell. 2007 129:289-301. [PubMed:17412408] [PMC:PMC2175179]

About FANCA

O15360 NM_000135 NP_000126.2

FANCA Antibody, FA1 Antibody, FACA Antibody, FA-H Antibody, Fanconi anemia group A protein Antibody, Fanconi anemia, type 1 Antibody, FA Antibody, Protein FACA Antibody, FAA Antibody, FANCH Antibody


Immunohistochemistry

Immunohistochemistry
Anti-FANCA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B70 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-FANCA Antibody - Western Blot. Western blot of affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301.

Western Blot

Western Blot
Anti-FANCA Antibody - Western Blot. Western blot of affinity purified anti-FANCA antibody shows detection of a band at ~133 kD (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1500. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye800 fluorescence images were captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 9/17/2014

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