Human F8 / FVIII / Factor VIII

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Caprylic acid and ammonium sulfate precipitations and solid-phase adsorption

Unmodified

Human Factor VIIIC (F. VIII) purified from F. VIII concentrate.

Recognizes human F. VIII as demonstrated by immunodiffusion and ELISA. No reactivity was observed with von Willebrand Factor.

• IHC
• IHC - Paraffin (5 µg/ml)
• ELISA

Immunohistochemistry: LS-B2979 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2979 was determined to be 5 ug/ml.

10 mM HEPES, pH 7.4, 0.15 M NaCl, 50% Glycerol

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P00451 NCBI: NM_000132 NP_000123.1