Human F8 / FVIII / Factor VIII

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Rat, Dog

IgG1 Monoclonal

RFF-VIIIC/10

Unconjugated

Protein G purified

Unmodified

Affinity purified human Factor VIII.

Recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause hemophilia A, a disorder characterized by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Clone RFF-VIIIC/10 recognizes an epitope within the non-functional, middle domain of full-length Factor VIII.

• Western blot (1:100)
• ELISA
• Radioimmunoassay

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P00451 NCBI: NM_000132 NP_000123.1