Mouse Monoclonal (IgG2b,k) to Mouse EFNB1 / Ephrin B1
Western blot, ELISA
Mouse EFNB1 / Ephrin B1
Mouse, Rat (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1 - 3 µg/ml), ELISA (0.1 - 1 µg/ml)
Specificity and Use
EFNB1 / Ephrin B1 antibody was raised against synthetic peptide derived from the mid region of mouse ephrin-B1 protein.
This antibody recognizes mouse ephrin-B1 recombinant protein and exhibits no cross-reactivity with a related ephrin-B2 recombinant protein. On Western blots it identifies a band at ~65kD. Reactivity has been confirmed with a mouse ephrin-B1 recombinant protein. Based on amino acid sequence homology, cross-reactivity with rat is expected.
Suitable for use in Western Blot and ELISA. Western Blot: 1-3 ug/ml. ELISA: 0.1-1 ug/ml.
PBS, pH 7.4, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C