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Anti-EBI3 Antibody (clone DNT27) IHC-plus™ LS-B5764

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

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EBI3 Antibody LS-B5764 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-EBI3 antibody IHC of human lymphoid-dendritic cells.
Western Blot
Recombinant mouse EBI3/p28 (covalently linked IL-27) protein (lane 1) and mouse bone...

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Rat Monoclonal [clone DNT27] (IgG2a,k) to Mouse EBI3
Mouse, Human
IHC - Paraffin, Western blot
Unconjugated

Details

Mouse EBI3
Rat
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [DNT27]
Affinity purified

Applications

IHC - Paraffin (10 µg/ml), Western blot

Specificity and Use

EBI3 antibody was raised against mouse EBI3
This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. This can be used at less than or equal to 1-5 ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging

Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

About EBI3

Q14213 NM_005755 NP_005746.2

EBI3 Antibody, Epstein-Barr virus induced 3 Antibody, IL-27 subunit beta Antibody, IL27 subunit Antibody, IL27B Antibody, Interleukin-27 subunit beta Antibody, IL-27B Antibody, Cytokine receptor Antibody, EBV-induced gene 3 protein Antibody, IL35 subunit Antibody


Immunohistochemistry

Immunohistochemistry
Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5764 concentration 10 ug/ml.

Western Blot

Western Blot
Recombinant mouse EBI3/p28 (covalently linked IL-27) protein (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane3) were analyzed by SDS-PAGE followed by immunoblotting with 5 ug/ml of anti-mouse EBI3 (DNT27). Bands were visualized with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG.

Requested From: United States
Date Requested: 7/31/2014
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