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CYP27B1

cytochrome P450, family 27, subfamily B, polypeptide 1

CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Gene Name: cytochrome P450, family 27, subfamily B, polypeptide 1
Family/Subfamily: Cytochrome P450 , not assigned-Cytochrome P450
Synonyms: CYP27B1, 1alpha(OH)ase, 25-OHD-1 alpha-hydroxylase, Calcidiol 1-monooxygenase, CYP1alpha, CYP27B, Cytochrome P450C1 alpha, Cytochrome p450 27B1, Cytochrome P450VD1-alpha, p450c1, VD3 1A hydroxylase, PDDR, VDD1, CP2B, CYP1, VDDR, VDDRI
Target Sequences: NM_000785 NP_000776.1 O15528

Publications (1)

1
Hemoglobin alters vitamin carrier uptake and vitamin D metabolism in proximal tubule cells: implications for sickle cell disease. Megan L Gliozzi, Youssef Rbaibi, Kimberly R Long, Dario A Vitturi, Ora A Weisz. American journal of physiology. Cell physiology. 2019 November;317:C993-C1000. [Full Text Article] [PubMed:31509446] [PMC:PMC6879883] Related Antibodies: LS-C407761.

☰ Filters
Antibodies (34)
Proteins (8)
ELISA Kits (4)
Custom (1)
Over-Expression Lysate (2)
Primary (34)
Recombinant (6)
Sandwich (3)
CYP27B1 (46)
Human (19)
Mouse (30)
Rat (15)
IHC (6)
IHC-P (3)
WB (34)
Flo (1)
ELISA (5)
IF (1)
rabbit (33)
mouse (1)
IgG (6)
IgG1,k (1)
monoclonal mc (1)
polyclonal pc (33)
1981CT820.303.81 (1)
96-Well Strip Plate (4)
APC (3)
APC Conjugated (4)
Azide-free (1)
Biotin Conjugated (4)
Cy3 Conjugated (3)
Cy7 Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (4)
PE Conjugated (3)
Unconjugated (10)
aa251-500 (8)
aa256-508 (8)
aa258-504 (8)
aa399-429 (3)
C-Terminus (1)
aa475-508 (1)
aa482-508 (1)
No (45)
Yes (1)
Cell Lysates (1)
Tissue Homogenates (3)
6His, N-terminus (1)
GST, N-terminus (1)
His, N-Terminal (1)
His-T7 (3)
Myc-DDK (Flag) (2)
Human (4)
Mouse (2)
Rat (1)
293T Cells (1)
E. coli (5)
HEK 293 Cells (1)
Wheat Germ Extract (1)
Purified (1)
CYP27B1 Antibody - CYP27B1 antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
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CYP27B1 Rabbit anti-Human Polyclonal (aa475-508) Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
CYP27B1 Antibody - CYP27B1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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CYP27B1 Rabbit anti-Human Polyclonal (aa482-508) Antibody
Rat, Human
Flo, IHC, IHC-P, WB
Unconjugated
200 µl/$393
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
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CYP27B1 Rabbit anti-Rat Polyclonal (aa251-500) Antibody
Mouse, Rat
WB
Unconjugated
100 µl/$317; 200 µl/$363; 500 µl/$515; 1 ml/$591; 2 ml/$816; 5 ml/$1,331
CYP27B1 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His, N-terminus
1 mg/$1,679; 100 µg/$598; 20 µg/$405
CYP27B1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
56.3 kDa
20 µg/$215
CYP27B1 Antibody
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CYP27B1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100 µg/$575
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CYP27B1 Rabbit anti-Human Polyclonal (aa399-429) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$665
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CYP27B1 Rabbit anti-Human Polyclonal (aa399-429) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$665
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CYP27B1 Rabbit anti-Human Polyclonal (aa399-429) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$665
CYP27B1 Antibody - Immunofluorescence analysis of HeLa cells using CYP27B1 Polyclonal Antibody at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.
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CYP27B1 Rabbit anti-Human Polyclonal Antibody
Rat, Human
IF, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
CYP27B1 Antibody - Anti-CYP27B1 Antibody (C-term) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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CYP27B1 Mouse anti-Human Monoclonal (C-Terminus) (1981CT820.303.81) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$393
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CYP27B1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
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CYP27B1 Rabbit anti-Human Polyclonal (aa256-508) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
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CYP27B1 Rabbit anti-Human Polyclonal (aa256-508) (HRP) Antibody
Mouse, Human
WB
HRP Conjugated
100 µl/$338; 200 µl/$393; 500 µl/$575; 1 ml/$702; 2 ml/$939; 5 ml/$1,593
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
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CYP27B1 Rabbit anti-Human Polyclonal (aa256-508) (Biotin) Antibody
Mouse, Human
WB
Biotin Conjugated
100 µl/$319; 200 µl/$365; 500 µl/$519; 1 ml/$596; 2 ml/$824; 5 ml/$1,348
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
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CYP27B1 Rabbit anti-Human Polyclonal (aa256-508) (FITC) Antibody
Mouse, Human
WB
FITC Conjugated
100 µl/$348; 200 µl/$407; 500 µl/$605; 1 ml/$755; 2 ml/$993; 5 ml/$1,716
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
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CYP27B1 Rabbit anti-Human Polyclonal (aa256-508) (APC) Antibody
Mouse, Human
WB
APC Conjugated
100 µl/$407; 200 µl/$491; 500 µl/$850; 1 ml/$1,008; 2 ml/$1,308; 5 ml/$2,451
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
Select
CYP27B1 Rabbit anti-Human Polyclonal (aa256-508) (APC, Cy7) Antibody
Mouse, Human
WB
APC, Cy7 Conjugated
100 µl/$505; 200 µl/$648; 500 µl/$1,152; 1 ml/$1,401; 2 ml/$1,961; 5 ml/$3,676
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
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CYP27B1 Rabbit anti-Human Polyclonal (aa256-508) (Cy3) Antibody
Mouse, Human
WB
Cy3 Conjugated
100 µl/$407; 200 µl/$491; 500 µl/$850; 1 ml/$1,008; 2 ml/$1,308; 5 ml/$2,451
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
Select
CYP27B1 Rabbit anti-Human Polyclonal (aa256-508) (PE) Antibody
Mouse, Human
WB
PE Conjugated
100 µl/$407; 200 µl/$491; 500 µl/$850; 1 ml/$1,008; 2 ml/$1,308; 5 ml/$2,451
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
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CYP27B1 Rabbit anti-Mouse Polyclonal (aa258-504) Antibody
Mouse
WB
Unconjugated
100 µl/$311; 200 µl/$355; 500 µl/$499; 1 ml/$571; 2 ml/$777; 5 ml/$1,261
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
Select
CYP27B1 Rabbit anti-Mouse Polyclonal (aa258-504) (Biotin) Antibody
Mouse
WB
Biotin Conjugated
100 µl/$321; 200 µl/$369; 500 µl/$527; 1 ml/$611; 2 ml/$842; 5 ml/$1,387
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
Select
CYP27B1 Rabbit anti-Mouse Polyclonal (aa258-504) (APC) Antibody
Mouse
WB
APC Conjugated
100 µl/$412; 200 µl/$499; 500 µl/$869; 1 ml/$1,030; 2 ml/$1,345; 5 ml/$2,521
CYP27B1 Antibody - Western blot of recombinant CYP27B1.
Select
CYP27B1 Rabbit anti-Mouse Polyclonal (aa258-504) (PE) Antibody
Mouse
WB
PE Conjugated
100 µl/$412; 200 µl/$499; 500 µl/$869; 1 ml/$1,030; 2 ml/$1,345; 5 ml/$2,521
CYP27B1 Antibody - Western blot analysis of extracts of various cell lines, using CYP27B1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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CYP27B1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
Viewing 1-25 of 46 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).