Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (5 µg/ml), Immunoprecipitation
Specificity and Use
CTCF antibody was raised against a synthetic peptide mapping to a region between residues 150 and 200 of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664).
Species cross-reactivity: Human. Other species have not been tested. Reactivity with mouse (but not X. laevis) CTCF is expected owing to similarity of sequence between this ortholog and human CTCF at the site to which the epitope maps.
Immunohistochemistry: LS-B3184 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3184 was determined to be 5 mg/ml.