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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3474-50 | 50 µl | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human CRYAB / Alpha B Crystallin Antibody (clone 1D11C6E6, IHC, WB) LS‑B3474
IHC‑plus™ Monoclonal Mouse anti‑Human CRYAB / Alpha B Crystallin Antibody (clone 1D11C6E6, IHC, WB) LS‑B3474
Note: This antibody replaces LS-C41529, LS-C171398
Antibody:
CRYAB / Alpha B Crystallin Mouse anti-Human Monoclonal (1D11C6E6) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CRYAB / Alpha B Crystallin Mouse anti-Human Monoclonal (1D11C6E6) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Alpha B Crystallin antibody LS-B3474 is an unconjugated mouse monoclonal antibody to human Alpha B Crystallin (CRYAB). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CRYAB / Alpha B Crystallin
Synonyms
CRYAB | Alpha-crystallin B chain | CRYA2 | Crystallin, alpha B | CTPP2 | Heat shock protein beta-5 | HSPB5 | Alpha B Crystallin | Alpha(B)-crystallin | Rosenthal fiber component | Heat-shock 20 kD like-protein
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
1D11C6E6
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of CRYAB (aa1-175) expressed in E. coli.
Specificity
Human
Applications
- IHC
- IHC - Paraffin (1:200)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3474 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3474 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CRYAB / Alpha B Crystallin
Validation
Anti-CRYAB / Alpha B Crystallin antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CRYAB / Alpha B Crystallin antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CRYAB / Alpha B Crystallin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CRYAB / Alpha B Crystallin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CRYAB / Alpha B Crystallin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CRYAB / Alpha B Crystallin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ CRYAB / Alpha B Crystallin Antibody (clone 1D11C6E6) for IHC, WB/Western, ELISA LS-B3474 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Bovine, Pig
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Bovine, Dog, Pig, Sheep
Applications:
Western blot, Immunoprecipitation
Species:
Bovine, Human, Mouse, Rat, Chicken
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Bovine
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Reactivity:
Human
Range:
0.16-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
