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Anti-COL3A1 / Collagen III Antibody (aa1247-1389) LS-C186224

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.97 mg/ml $335

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COL3A1 / Collagen III Antibody LS-C186224 Images

[click image for a larger image and more details]
Immunohistochemistry
IHC of paraffin-embedded NCIN87 xenograft using Collagen III alpha1 antibody at 1:500 dilution.
ICC
Immunofluorescence of methanol-fixed HeLa using COL3A1 antibody at 1:50 dilution.
Western Blot
Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. COL3A1 antibody diluted at 1:1000.
 

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Rabbit Polyclonal (IgG) to Human COL3A1 / Collagen III
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human COL3A1 / Collagen III
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1:100 - 1:1000), ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)

Specificity and Use

COL3A1 / Collagen III antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 1247 and 1389 of Collagen III alpha1. Percent identity by BLAST analysis: Human (100%); Bovine (93%); Mouse, Rat (92%); Chicken (86%); Xenopus (81%).
aa1247-1389
Human COL3A1
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About COL3A1 / Collagen III

P02461 NM_000090 NP_000081.1

COL3A1 Antibody, Alpha1 (III) collagen Antibody, Collagen alpha 1(iii) Antibody, Collagen alpha-1(III) chain Antibody, Collagen, type III, alpha 1 Antibody, Collagen, fetal Antibody, EDS4A Antibody

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded NCIN87 xenograft using Collagen III alpha1 antibody at 1:500 dilution.

ICC

ICC
Immunofluorescence of methanol-fixed HeLa using COL3A1 antibody at 1:50 dilution.

Western Blot

Western Blot
Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. COL3A1 antibody diluted at 1:1000.
 

Requested From: United States
Date Requested: 8/20/2014

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