Human COL1A1 / Collagen I Alpha 1

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Affinity purified

Unmodified

Peptide from the human collagen I alpha 1 propeptide sequence

Specific for the propeptide portion of the ~180 kDa collagen I alpha 1 polypeptide in human lung fibroblast extract.

• IHC (1:100)
• Western blot (1:1000)

The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.

10 mM HEPES, pH 7.5, 150 mM NaCl, 50% Glycerol, 100 ug/ml BSA

Store at -20°C, stable for 1 year. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02452 NCBI: NM_000088 NP_000079.2