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Catalog Number Size Price
LS-C34669-100 100 µg (0.5 mg/ml) $503 
CHAT Antibody - Chat (mouse) Antibody (1:2000) staining of PFA-perfused cryosection of Mouse Forebrain. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Éva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.
CHAT Antibody - CHAT antibody (0.2 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CHAT Antibody - Chat antibody (1µg/ml) staining of Mouse (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CHAT Antibody - Chat (mouse) Antibody (1:2000) staining of PFA-perfused cryosection of Mouse Forebrain. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Éva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.
CHAT Antibody - CHAT antibody (0.2 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CHAT Antibody - Chat antibody (1µg/ml) staining of Mouse (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Polyclonal Goat anti‑Mouse CHAT Antibody (aa629‑642, IHC, WB) LS‑C34669

Polyclonal Goat anti‑Mouse CHAT Antibody (aa629‑642, IHC, WB) LS‑C34669

Note: This antibody replaces LS-C79269
Antibody:
CHAT Goat anti-Mouse Polyclonal (aa629-642) Antibody
Application:
IHC-Fr, WB, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C34669-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CHAT Goat anti-Mouse Polyclonal (aa629-642) Antibody
Application:
IHC-Fr, WB, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CHAT antibody LS-C34669 is an unconjugated goat polyclonal antibody to mouse CHAT (aa629-642). Validated for IHC, Peptide-ELISA and WB.
Target
Mouse CHAT
Synonyms
CHAT | Choline acetylase | Choline acetyltransferase | Choline O-acetyltransferase | CMS1A2 | CHOACTASE | CMS1A
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KERARGPTKPSNLD, from the C-Terminus of protein sequence according to NP_034021.1.
Epitope
aa629-642
Specificity
Mouse CHAT. This antibody is NOT expected to cross-react to Human CHAT.
Applications
  • IHC - Frozen (1:2000)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75-80 kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3 kDa according to NP_034021.1). Recommended concentration: 0.2-0.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHAT
P28329 NM_020549 NP_065574.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024