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Anti-CENPQ Antibody LS-C154023

Ordering

Wt. Vol. Conc. Price
100 µg - - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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CENPQ Antibody LS-C154023 Images

[click image for a larger image and more details]
Immunofluorescence
Anti-CENP-Q Antibody - Immunofluorescence Microscopy.
Western Blot
Anti-CENP-Q Antibody - Western Blot.

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Rabbit Polyclonal (IgG) to Human CENPQ
Human
ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human CENPQ
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified

Applications

ICC, Immunofluorescence, Western blot (1:100 - 1:500), ELISA

Specificity and Use

CENPQ antibody was raised against this protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein.
This antibody is specific for human CENP-Q protein. A BLAST analysis of the full length sequence was used to suggest partial cross-reactivity with CENP-Q based on the following percentage homologies: macaque (91%), horse (76%), bovine (74%), dog (73%), swine (71%), mouse (65%) and rat (60%). Cross-reactivity with CENP-Q from other sources has not been determined.
This protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26-31 kD in size corresponding to human CENP-Q by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CENPQ

Q7L2Z9 NM_018132 NP_060602.2

CENPQ Antibody, C6orf139 Antibody, Centromere protein Q Antibody, CENP-Q Antibody


Immunofluorescence

Immunofluorescence
Anti-CENP-Q Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.

Western Blot

Western Blot
Anti-CENP-Q Antibody - Western Blot. Western blot of protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5ug/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.

Requested From: United States
Date Requested: 9/18/2014

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