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| Catalog Number | Size | Price |
|---|---|---|
| LS-C17121-50 | 50 µg | $786 |
Polyclonal Rabbit anti‑Human CDKN1B / p27 Kip1 Antibody (phospho‑Ser10, IF, WB) LS‑C17121
Polyclonal Rabbit anti‑Human CDKN1B / p27 Kip1 Antibody (phospho‑Ser10, IF, WB) LS‑C17121
Antibody:
CDKN1B / p27 Kip1 Rabbit anti-Human Polyclonal (pSer10) Antibody
Application:
IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CDKN1B / p27 Kip1 Rabbit anti-Human Polyclonal (pSer10) Antibody
Application:
IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
P27 Kip1 antibody LS-C17121 is an unconjugated rabbit polyclonal antibody to p27 Kip1 (CDKN1B) (pSer10) from human. It is reactive with human, mouse and rat. Validated for IF, IP and WB.
Target
Human CDKN1B / p27 Kip1
Synonyms
CDKN1B | KIP1 | MEN1B | P27 Kip1 | p27KIP1 | MEN4 | CDKN4
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.
Epitope
pSer10
Specificity
Recognizes p27 when it is phosphorylated at the Ser10 residue. Lysates of HEK 293T cells transfected with an expression plasmid encoding a p27 mutant where Ser10 was substituted with an alanine residue were used to confirm the antibody's specificity. Reactivity was confirmed with HEK293 cells expressing wild-type p27/KIP that is normally phosphorylated. NIH3T3 and MCF-7 cell lysates can also be used as positive controls. Species cross-reactivity: Human, rat and mouse.
Applications
- Immunofluorescence (5 - 10 µg/ml)
- Western blot (1 - 3 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1-3 ug/ml. Immunoprecipitation: 5-10 ug/IP reaction. Immunofluorescence: 5-10 ug/ml.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CDKN1B / p27 Kip1
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Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Human, Mouse, Rat
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
