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Anti-CDH1 / E Cadherin Antibody (clone 3F4) IHC-plus™ LS-B4297

Ordering

Wt. Vol. Conc. Price
50 µg - 0.4 mg/ml $450

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CDH1 / E Cadherin Antibody LS-B4297 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-E Cadherin antibody IHC of human, spleen.
Immunohistochemistry
Anti-E Cadherin antibody IHC of human lung.
Western Blot
Western blot of CDH1 expression in transfected 293T cell line by CDH1 monoclonal antibody, clone...
Proximity Ligation Analysis
Proximity Ligation Analysis (PLA) of protein-protein interactions between EGFR and CDH1 HeLa...

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Mouse Monoclonal [clone 3F4] (IgG1,k) to Human CDH1 / E Cadherin
Human
IHC - Paraffin, Western blot, ELISA, Proximity Ligation Assay
Unconjugated

Details

Human CDH1 / E Cadherin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3F4]
Protein A purified

Applications

IHC - Paraffin (3 µg/ml), Western blot, ELISA, Proximity Ligation Assay

Specificity and Use

CDH1 / E Cadherin antibody was raised against cDH1 (NP_004351, 381 a.a. ~ 481 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Transfected lysate) - positive control transfected 293T cell line. In situ Proximity Ligation Assay (Cell).

Packaging

PBS, pH 7.4.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About CDH1 / E Cadherin

P12830 NM_004360 NP_004351.1

CDH1 Antibody, CD324 Antibody, CDHE Antibody, Cell-CAM 120/80 Antibody, E Cadherin Antibody, E-Cadherin Antibody, ECAD Antibody, Epithelial cadherin Antibody, Arc-1 Antibody, UVO Antibody, Cadherin-1 Antibody, CAM 120/80 Antibody, CD324 antigen Antibody, LCAM Antibody, Uvomorulin Antibody

E Cadherin (CDH1) is a classical cadherin from the cadherin superfamily. It is a calcium dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis.

Immunohistochemistry

Immunohistochemistry
Anti-E Cadherin antibody IHC of human, spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4297 concentration 3 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-E Cadherin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4297 concentration 3 ug/ml.

Western Blot

Western Blot
Western blot of CDH1 expression in transfected 293T cell line by CDH1 monoclonal antibody, clone 3F4.

Proximity Ligation Analysis

Proximity Ligation Analysis
Proximity Ligation Analysis (PLA) of protein-protein interactions between EGFR and CDH1 HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-CDH1 mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection Ki.

Requested From: United States
Date Requested: 10/31/2014

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