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Anti-CCNL1 / Cyclin L1 Antibody (aa314-369) IHC-plus™ LS-B79

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395

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CCNL1 / Cyclin L1 Antibody LS-B79 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CCNL1 / Cyclin L1 antibody IHC of human thymus.
Western Blot
Western blot of Protein A Purified anti-Cyclin L1a antibody shows detection of a band ~59 kDa...

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Rabbit Polyclonal to Human CCNL1 / Cyclin L1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human CCNL1 / Cyclin L1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Protein A purified

Applications

IHC - Paraffin (10 - 20 µg/ml), Western blot (1:2000 - 1:5000), Immunoprecipitation, ELISA (1:5000 - 1:25000)

Specificity and Use

CCNL1 / Cyclin L1 antibody was raised against synthetic polypeptide corresponding to amino acids 314-369 of Human Cyclin L1a protein.
aa314-369
Human Cyclin L1a
Immunohistochemistry: LS-B79 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B79 was determined to be 10-20 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
50 µl
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

Publications (1)

1
Role of CDK/cyclin complexes in transcription and RNA splicing. Loyer P, Trembley JH, Katona R, Kidd VJ, Lahti JM. Cellular signalling. 2005 17:1033-51. [PubMed:15935619]

About CCNL1 / Cyclin L1

Q9UK58 NM_020307 NP_064703.1

CCNL1 Antibody, ANIA6A Antibody, Ania-6a Antibody, BM-001 Antibody, Cyclin L ania-6a Antibody, Cyclin L gamma Antibody, Cyclin L1 Antibody, Cyclin-L Antibody, Cyclin-L1 Antibody, PRO1073 Antibody

Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Immunohistochemistry

Immunohistochemistry
Anti-CCNL1 / Cyclin L1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B79 concentration 20 ug/ml.

Western Blot

Western Blot
Western blot of Protein A Purified anti-Cyclin L1a antibody shows detection of a band ~59 kDa corresponding to a Cyclin L1a (arrowhead) present in mouse brain whole cell lysate. Approximately 35g of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 8/23/2014

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