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Anti-CBLC Antibody (aa444-458) IHC-plus™ LS-B208

Ordering

Wt. Vol. Conc. Price
50 µg - 1.42 mg/ml $395

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CBLC Antibody LS-B208 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CBLC antibody IHC of human pancreas.
Western Blot
Anti-Cbl-c Antibody - Immunoprecipitation and Western Blot.

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Rabbit Polyclonal to Human CBLC
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human CBLC
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Immunofluorescence, Western blot (1:500 - 1:3000), Immunoprecipitation, ELISA (1:10000 - 1:50000)

Specificity and Use

CBLC antibody was raised against synthetic peptide from human CBLC.
aa444-458
Amino acids 444-458 of Human Cbl-c.
Immunohistochemistry: LS-B208 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B208 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CBLC

Q9ULV8 NM_012116 NP_036248.3

CBLC Antibody, CBL-3 Antibody, CBL3 Antibody, RNF57 Antibody, SH3-binding protein CBL-C Antibody, SH3-binding protein CBL-3 Antibody, CBL-c Antibody, CBL-SL Antibody, RING finger protein 57 Antibody

CBLC belongs to a family of ubiquitin ligases that are negative regulators of tyrosine kinase signaling downstream of receptor tyrosine kinase and lymphocyte antigen receptors. Monoubiquitin tag promotes sorting of activated receptor and associated proteins into internal vesicles facilitating their lysosomal degradation, while polyubiquitin tag promotes proteasomal degradation. CBLC is expressed in epithelial cells.

Immunohistochemistry

Immunohistochemistry
Anti-CBLC antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B208 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-Cbl-c Antibody - Immunoprecipitation and Western Blot. Immunoprecipitation and Western blot of Affinity Purified anti-Cbl-c antibody shows detection of a pre-dominant band at ~52 kD corresponding to Cbl-c (arrowhead) in transfected cell lysates (left panel). Lysates are from Hek 293T cells transfected with empty vector or with Cbl-c. The predicted size of Cbl-c is 52 kD. Size markers in kD are shown to the left of the panel. The right panel shows western blotting after first immunoprecipitating with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody. Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.

Requested From: United States
Date Requested: 7/28/2014
Copyright © 2014 LifeSpan BioSciences, Inc. All Rights Reserved