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Catalog Number Size Price
LS-C154780-100 100 µg (0.5 mg/ml) $503 
CASP3 / Caspase 3 Antibody - Caspase 3 Antibody Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
CASP3 / Caspase 3 Antibody - CASP3 antibody (1 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CASP3 / Caspase 3 Antibody - CASP3 / Caspase 3 antibody (0.5µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CASP3 / Caspase 3 Antibody - Caspase 3 Antibody Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
CASP3 / Caspase 3 Antibody - CASP3 antibody (1 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CASP3 / Caspase 3 Antibody - CASP3 / Caspase 3 antibody (0.5µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Polyclonal Goat anti‑Human CASP3 / Caspase 3 Antibody (aa101‑112, IF, WB) LS‑C154780

Polyclonal Goat anti‑Human CASP3 / Caspase 3 Antibody (aa101‑112, IF, WB) LS‑C154780

Antibody:
CASP3 / Caspase 3 Goat anti-Human Polyclonal (aa101-112) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C154780-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CASP3 / Caspase 3 Goat anti-Human Polyclonal (aa101-112) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 3 antibody LS-C154780 is an unconjugated goat polyclonal antibody to Caspase 3 (CASP3) (aa101-112) from human. It is reactive with human and hamster. Validated for IF, Peptide-ELISA and WB.
Target
Human CASP3 / Caspase 3
Synonyms
CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
Host
Goat
Reactivity
Human, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-RDVSKEDHSKRS, from the internal region of the protein sequence according to NP_004337.2NP_116786.1.
Epitope
aa101-112
Specificity
Human Caspase 3. This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 30kD band observed in lysates of cell line MOLT4 (calculated MW of 31.6kD according to NP_004337.2;). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 65kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP3 / Caspase 3
P42574 NM_004346 NP_004337.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024