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Anti-BRAF / B-Raf Antibody (phospho-Thr598/Ser601) IHC-plus™ LS-B1627

Ordering

Wt. Vol. Conc. Price
- 50 µl 0.5 mg/ml $395

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BRAF / B-Raf Antibody LS-B1627 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-BRAF antibody IHC of human testis.
ICC
Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against...

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Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Human
IHC - Paraffin, Immunofluorescence
Unconjugated

Details

Human BRAF / B-Raf
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1:100), Immunofluorescence

Specificity and Use

BRAF / B-Raf antibody was raised against synthetic peptide from human BRAF.
pThr598/Ser601
Modified peptide
Immunohistochemistry: LS-B1627 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1627 was determined to be 1:100.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About BRAF / B-Raf

P15056 NM_004333 NP_004324.2

BRAF Antibody, 94 kDa B-raf protein Antibody, B-raf Antibody, BRAF1 Antibody, B Raf Antibody, Proto-oncogene B-Raf Antibody, p94 Antibody, B-RAF1 Antibody, NS7 Antibody, ONCOGENE BRAF Antibody, RAFB1 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1627 dilution 1:100.

ICC

ICC
Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against Thr598/Ser601 phosphorylated B-Raf, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.

Requested From: United States
Date Requested: 9/2/2014

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