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Anti-BRAF / B-Raf Antibody (clone 1H12) IHC-plus™ LS-B3862

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

BRAF / B-Raf Antibody LS-B3862 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-BRAF antibody IHC of human brain, cortex.
Immunohistochemistry
Anti-BRAF antibody IHC of human testis.
Immunohistochemistry
Anti-BRAF antibody IHC of human brain, cerebellum.
Immunofluorescence
Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using anti-BRAF mAb (green).
Western Blot
Western blot of BRAF monoclonal antibody against HeLa (1), HL60 (2), HepG2(3) and NIH/3T3 (4)...
 

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Mouse Monoclonal [clone 1H12] (IgG1) to Human BRAF / B-Raf
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human BRAF / B-Raf
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1H12]
Ascites

Applications

IHC - Paraffin (1:200 - 1:300), Immunofluorescence (1:200 - 1:1000), Western blot (1:500 - 1:2000), ELISA (1:10000)

Specificity and Use

BRAF / B-Raf antibody was raised against purified recombinant fragment of human BRAF (aa 285-401) expressed in E. coli
Human BRAF
Immunohistochemistry: LS-B3861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3861 was determined to be 1:200.

Packaging

Ascites fluid, 0.03% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BRAF / B-Raf

P15056 NM_004333 NP_004324.2

BRAF Antibody, 94 kDa B-raf protein Antibody, B-raf Antibody, BRAF1 Antibody, B Raf Antibody, Proto-oncogene B-Raf Antibody, p94 Antibody, B-RAF1 Antibody, NS7 Antibody, ONCOGENE BRAF Antibody, RAFB1 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3862 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3862 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3862 dilution 1:200.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using anti-BRAF mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Western Blot

Western Blot
Western blot of BRAF monoclonal antibody against HeLa (1), HL60 (2), HepG2(3) and NIH/3T3 (4) cell lysate.
 

Requested From: United States
Date Requested: 10/31/2014

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