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This antibody may not be available in some countries due to import restrictions. Please inquire before purchase.

Anti-BMI1 / PCGF4 Antibody (aa252-264) IHC-plus™ LS-B346

Ordering

Wt. Vol. Conc. Price
50 µg - 0.91 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

BMI1 / PCGF4 Antibody LS-B346 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-BMI-1 antibody IHC of human skeletal muscle.
Immunofluorescence
Anti-Bmi1 Antibody - Immunofluorescence Microscopy.
Western Blot
Anti-Bmi1 Antibody - Western Blot.
 

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Goat Polyclonal to Human BMI1 / PCGF4
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human BMI1 / PCGF4
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (2.5 µg/ml), ICC, Immunofluorescence, Western blot (1:500 - 1:3000), ELISA (1:5000 - 1:30000)

Specificity and Use

BMI1 / PCGF4 antibody was raised against synthetic peptide from human BMI1.
aa252-264
Amino acids 252-264 of human Bmi1 protein.
Immunohistochemistry: LS-B346 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B346 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About BMI1 / PCGF4

P35226 NM_005180 NP_005171.4

BMI1 Antibody, BMI-1 Antibody, Flvi-2/bmi-1 Antibody, FLVI2/BMI1 Antibody, PCGF4 Antibody, Polycomb group protein Bmi1 Antibody, Polycomb complex protein BMI-1 Antibody, Dna-binding protein bmi-1 Antibody, Polycomb group ring finger 4 Antibody, RING finger protein 51 Antibody, RNF51 Antibody



BMI1 / PCGF4 Antibodies (57)

Target Reactivity Applications Host Clonality Clone Format Epitope Publications
BMI1 / PCGF4 Rabbit Monoclonal EPR3745(2) Unconjugated Human, Rat IHC-P, ICC, IF, WB Internal No
Anti-BMI1 / PCGF4 Antibody (Internal) [clone EPR3745(2)] IHC-plus™ LS-B8010
IHC Image
IF Image
Rabbit Monoclonal [clone EPR3745(2)] (IgG) to Human BMI1 / PCGF4
Human, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal 3E3 Unconjugated Human IHC, ICC, IF, WB, Flo, ELISA No
Anti-BMI1 / PCGF4 Antibody [clone 3E3] LS-C169434
IHC Image
IHC Image
Mouse Monoclonal [clone 3E3] (IgG1) to Human BMI1 / PCGF4
Human
IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
BMI1 / PCGF4 Mouse-Balb/c Monoclonal 229F6 Unconjugated Human, Mouse, Rabbit IHC-Fr, WB, IP No
Anti-BMI1 / PCGF4 Antibody [clone 229F6] LS-C150548
Mouse-Balb/c Monoclonal [clone 229F6] (IgG1)
Human, Mouse, Rabbit
IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat ICC, WB Internal No
Anti-BMI1 / PCGF4 Antibody (Internal) LS-C19577
IF Image
ICC Image
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Mouse, Rat
ICC, Western blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human IHC-P, IF, WB, Flo No
Anti-BMI1 / PCGF4 Antibody LS-C156830
IHC Image
IF Image
Rabbit Polyclonal to Human BMI1 / PCGF4
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Mouse IHC-P, WB C-Terminus No
Anti-BMI1 / PCGF4 Antibody (C-Terminus) LS-C98479
WB Image
WB Image
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal FITC Conjugated Human IHC, WB, ELISA No
Anti-BMI1 / PCGF4 Antibody (FITC) LS-C211562
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
IHC, Western blot, ELISA
FITC Conjugated
BMI1 / PCGF4 Rabbit Polyclonal Biotin Conjugated Human IHC, WB, ELISA No
Anti-BMI1 / PCGF4 Antibody (Biotin) LS-C211563
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
IHC, Western blot, ELISA
Biotin Conjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human IHC, WB, ELISA No
Anti-BMI1 / PCGF4 Antibody LS-C211559
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
IHC, Western blot, ELISA
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal HRP Conjugated Human IHC, WB, ELISA No
Anti-BMI1 / PCGF4 Antibody (HRP) LS-C211561
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
IHC, Western blot, ELISA
HRP Conjugated
BMI1 / PCGF4 Rabbit Polyclonal FITC Conjugated Human, Mouse IHC, WB, ELISA C-Terminus No
Anti-BMI1 / PCGF4 Antibody (C-Terminus) (FITC) LS-C224912
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human, Mouse
IHC, Western blot, ELISA
FITC Conjugated
BMI1 / PCGF4 Rabbit Polyclonal PE Conjugated Human, Mouse IHC, WB, ELISA C-Terminus No
Anti-BMI1 / PCGF4 Antibody (C-Terminus) (PE) LS-C224913
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human, Mouse
IHC, Western blot, ELISA
PE Conjugated
BMI1 / PCGF4 Rabbit Polyclonal AP Conjugated Human, Mouse IHC, WB, ELISA C-Terminus No
Anti-BMI1 / PCGF4 Antibody (C-Terminus) (AP) LS-C224910
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human, Mouse
IHC, Western blot, ELISA
AP Conjugated
BMI1 / PCGF4 Rabbit Polyclonal APC Conjugated Human, Mouse IHC, WB, ELISA C-Terminus No
Anti-BMI1 / PCGF4 Antibody (C-Terminus) (APC) LS-C224911
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human, Mouse
IHC, Western blot, ELISA
APC Conjugated
BMI1 / PCGF4 Rabbit Polyclonal FITC Conjugated Human IHC, ELISA aa128-158 No
Anti-BMI1 / PCGF4 Antibody (aa128-158) (FITC) LS-C221539
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
IHC, ELISA
FITC Conjugated
BMI1 / PCGF4 Rabbit Polyclonal PE Conjugated Human IHC, ELISA aa128-158 No
Anti-BMI1 / PCGF4 Antibody (aa128-158) (PE) LS-C221541
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
IHC, ELISA
PE Conjugated
BMI1 / PCGF4 Rabbit Polyclonal AP Conjugated Human IHC, ELISA aa128-158 No
Anti-BMI1 / PCGF4 Antibody (aa128-158) (AP) LS-C221537
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
IHC, ELISA
AP Conjugated
BMI1 / PCGF4 Rabbit Polyclonal APC Conjugated Human IHC, ELISA aa128-158 No
Anti-BMI1 / PCGF4 Antibody (aa128-158) (APC) LS-C221538
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
IHC, ELISA
APC Conjugated
BMI1 / PCGF4 Mouse Monoclonal 4E10-1C5 Unconjugated Human WB, ELISA No
Anti-BMI1 / PCGF4 Antibody [clone 4E10-1C5] LS-C196758
WB Image
WB Image
Mouse Monoclonal [clone 4E10-1C5] (IgG1,k) to Human BMI1 / PCGF4
Human
Western blot, ELISA
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal LLBmi1-1 Unconjugated Human, Mouse ICC, WB, IP No
Anti-BMI1 / PCGF4 Antibody [clone LLBmi1-1] LS-C180218
ICC Image
WB Image
Mouse Monoclonal [clone LLBmi1-1] (IgG2b,k) to Human BMI1 / PCGF4
Human, Mouse
ICC, Western blot, Immunoprecipitation
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal Unconjugated Human IF, WB No
Anti-BMI1 / PCGF4 Antibody LS-C156831
ICC Image
WB Image
Mouse Monoclonal (IgG1,k) to Human BMI1 / PCGF4
Human
Immunofluorescence, Western blot
Unconjugated
BMI1 / PCGF4 Goat Polyclonal Unconjugated Human ICC, WB, Flo No
Anti-BMI1 / PCGF4 Antibody LS-C149862
ICC Image
Goat Polyclonal (IgG) to Human BMI1 / PCGF4
Human
ICC, Western blot, Flow Cytometry
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Chimpanzee, Mouse, Rat, Macaque WB aa270-320 No
Anti-BMI1 / PCGF4 Antibody (aa270-320) LS-C148527
WB Image
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Chimpanzee, Mouse, Rat, Macaque
Western blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat, Bovine, Cat, Rabbit, Chicken, Zebrafish, Primate WB aa1-100 No
Anti-BMI1 / PCGF4 Antibody (aa1-100) LS-C49129
WB Image
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Mouse, Rat, Bovine, Cat, Rabbit, Chicken, Zebrafish, Primate
Western blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Orangutan, Bovine, Dog WB C-Terminus No
Anti-BMI1 / PCGF4 Antibody (C-Terminus) LS-C30108
WB Image
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human, Orangutan, Bovine, Dog
Western blot
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal Unconjugated Human WB No
Anti-BMI1 / PCGF4 Antibody LS-C156832
WB Image
Mouse Monoclonal (IgG1,k) to Human BMI1 / PCGF4
Human
Western blot
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal Unconjugated Mouse WB No
Anti-BMI1 / PCGF4 Antibody LS-C164303
WB Image
Mouse Monoclonal (IgG2b) to Mouse BMI1 / PCGF4
Mouse
Western blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Mouse WB Internal No
Anti-BMI1 / PCGF4 Antibody (Internal) LS-C146455
WB Image
Rabbit Polyclonal to Mouse BMI1 / PCGF4
Mouse
Western blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat ICC, WB, ELISA Internal No
Anti-BMI1 / PCGF4 Antibody (Internal) LS-C209300
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Mouse, Rat
ICC, Western blot, ELISA
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal LLBmi1-1 HRP Conjugated Human, Mouse ICC, WB No
Anti-BMI1 / PCGF4 Antibody [clone LLBmi1-1] (HRP) LS-C181008
Mouse Monoclonal [clone LLBmi1-1] (IgG2b,k) to Human BMI1 / PCGF4
Human, Mouse
ICC, Western blot
HRP Conjugated
BMI1 / PCGF4 Mouse Monoclonal LLBmi1-1 DY550 Conjugated Human, Mouse ICC, WB No
Anti-BMI1 / PCGF4 Antibody [clone LLBmi1-1] (DY550) LS-C181009
Mouse Monoclonal [clone LLBmi1-1] (IgG2b,k) to Human BMI1 / PCGF4
Human, Mouse
ICC, Western blot
DY550 Conjugated
BMI1 / PCGF4 Mouse Monoclonal LLBmi1-1 DY488 Conjugated Human, Mouse ICC, WB No
Anti-BMI1 / PCGF4 Antibody [clone LLBmi1-1] (DY488) LS-C181007
Mouse Monoclonal [clone LLBmi1-1] (IgG2b,k) to Human BMI1 / PCGF4
Human, Mouse
ICC, Western blot
DY488 Conjugated
BMI1 / PCGF4 Mouse Monoclonal LLBmi1-1 Biotin Conjugated Human, Mouse ICC, WB No
Anti-BMI1 / PCGF4 Antibody [clone LLBmi1-1] (Biotin) LS-C181005
Mouse Monoclonal [clone LLBmi1-1] (IgG2b,k) to Human BMI1 / PCGF4
Human, Mouse
ICC, Western blot
Biotin Conjugated
BMI1 / PCGF4 Mouse Monoclonal LLBmi1-1 DY650 Conjugated Human, Mouse ICC, WB No
Anti-BMI1 / PCGF4 Antibody [clone LLBmi1-1] (DY650) LS-C181006
Mouse Monoclonal [clone LLBmi1-1] (IgG2b,k) to Human BMI1 / PCGF4
Human, Mouse
ICC, Western blot
DY650 Conjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Mouse WB Internal No
Anti-BMI1 / PCGF4 Antibody (Internal) LS-C141369
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Mouse
Western blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Mouse WB Internal No
Anti-BMI1 / PCGF4 Antibody (Internal) LS-C141368
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Mouse
Western blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Mouse WB Internal No
Anti-BMI1 / PCGF4 Antibody (Internal) LS-C141371
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Mouse
Western blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal HRP Conjugated Human, Mouse WB Internal No
Anti-BMI1 / PCGF4 Antibody (Internal) (HRP) LS-C141370
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Mouse
Western blot
HRP Conjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Gorilla, Orangutan, Gibbon, Monkey, Bat, Horse, Pig ELISA aa142-159 No
Anti-BMI1 / PCGF4 Antibody (aa142-159) LS-C148975
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Gorilla, Orangutan, Gibbon, Monkey, Bat, Horse, Pig
ELISA
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal 2704C2a Unconjugated Human DB No
Anti-BMI1 / PCGF4 Antibody [clone 2704C2a] LS-C184479
Mouse Monoclonal [clone 2704C2a] (IgG1) to Human BMI1 / PCGF4
Human
Dot Blot
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal Biotin Conjugated Human, Mouse WB Internal No
Anti-BMI1 / PCGF4 Antibody (Internal) (Biotin) LS-C141367
Rabbit Polyclonal to Human BMI1 / PCGF4
Human, Mouse
Western blot
Biotin Conjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Rat, Human, Mouse, Bovine, Cat, Dog WB No
Anti-BMI1 / PCGF4 Antibody LS-C147042
Rabbit Polyclonal to Rat BMI1 / PCGF4
Rat, Human, Mouse, Bovine, Cat, Dog
Western blot
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal 282CT3.7.6 APC Conjugated Human ICC, WB, ELISA No
Anti-BMI1 / PCGF4 Antibody [clone 282CT3.7.6] (APC) LS-C221257
Mouse Monoclonal [clone 282CT3.7.6] (IgG1,k) to Human BMI1 / PCGF4
Human
ICC, Western blot, ELISA
APC Conjugated
BMI1 / PCGF4 Mouse Monoclonal 282CT3.7.6 FITC Conjugated Human ICC, WB, ELISA No
Anti-BMI1 / PCGF4 Antibody [clone 282CT3.7.6] (FITC) LS-C221258
Mouse Monoclonal [clone 282CT3.7.6] (IgG1,k) to Human BMI1 / PCGF4
Human
ICC, Western blot, ELISA
FITC Conjugated
BMI1 / PCGF4 Mouse Monoclonal 282CT3.7.6 PE Conjugated Human ICC, WB, ELISA No
Anti-BMI1 / PCGF4 Antibody [clone 282CT3.7.6] (PE) LS-C221259
Mouse Monoclonal [clone 282CT3.7.6] (IgG1,k) to Human BMI1 / PCGF4
Human
ICC, Western blot, ELISA
PE Conjugated
BMI1 / PCGF4 Goat Polyclonal Unconjugated Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse IF, WB, ELISA aa252-254 No
Anti-BMI1 / PCGF4 Antibody (aa252-254) LS-C77104
Goat Polyclonal (IgG) to Human BMI1 / PCGF4
Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse
Immunofluorescence, Western blot, ELISA
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal Unconjugated Mouse, Human, Rat, Rabbit ICC, WB, IP aa1-202 No
Anti-BMI1 / PCGF4 Antibody (aa1-202) LS-C4191
Mouse Monoclonal (IgG1) to Mouse BMI1 / PCGF4
Mouse, Human, Rat, Rabbit
ICC, Western blot, Immunoprecipitation
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal 282CT3.7.6 AP Conjugated Human ICC, WB, ELISA No
Anti-BMI1 / PCGF4 Antibody [clone 282CT3.7.6] (AP) LS-C221255
Mouse Monoclonal [clone 282CT3.7.6] (IgG1,k) to Human BMI1 / PCGF4
Human
ICC, Western blot, ELISA
AP Conjugated
BMI1 / PCGF4 Rabbit Polyclonal PE Conjugated Human WB, ELISA No
Anti-BMI1 / PCGF4 Antibody (PE) LS-C224918
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
Western blot, ELISA
PE Conjugated
BMI1 / PCGF4 Mouse Monoclonal Unconjugated Human WB, ELISA No
Anti-BMI1 / PCGF4 Antibody LS-C4192
Mouse Monoclonal (IgG1,k) to Human BMI1 / PCGF4
Human
Western blot, ELISA
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal Unconjugated Mouse, Human WB, IP No
Anti-BMI1 / PCGF4 Antibody LS-C125189
Mouse Monoclonal (IgG1,k) to Mouse BMI1 / PCGF4
Mouse, Human
Western blot, Immunoprecipitation
Unconjugated
BMI1 / PCGF4 Rabbit Polyclonal AP Conjugated Human WB, ELISA No
Anti-BMI1 / PCGF4 Antibody (AP) LS-C224914
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
Western blot, ELISA
AP Conjugated
BMI1 / PCGF4 Rabbit Polyclonal APC Conjugated Human WB, ELISA No
Anti-BMI1 / PCGF4 Antibody (APC) LS-C224915
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
Western blot, ELISA
APC Conjugated
BMI1 / PCGF4 Rabbit Polyclonal FITC Conjugated Human WB, ELISA No
Anti-BMI1 / PCGF4 Antibody (FITC) LS-C224916
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human
Western blot, ELISA
FITC Conjugated
BMI1 / PCGF4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat WB No
Anti-BMI1 / PCGF4 Antibody LS-C4194
Rabbit Polyclonal (IgG) to Human BMI1 / PCGF4
Human, Mouse, Rat
Western blot
Unconjugated
BMI1 / PCGF4 Mouse Monoclonal Unconjugated Human WB No
Anti-BMI1 / PCGF4 Antibody LS-C77105
Mouse Monoclonal (IgG1) to Human BMI1 / PCGF4
Human
Western blot
Unconjugated
BMI1 / PCGF4 Goat Polyclonal Unconjugated Human IHC-P, ICC, IF, WB, ELISA aa252-264 No
Anti-BMI1 / PCGF4 Antibody (aa252-264) IHC-plus™ LS-B346
IHC Image
IF Image
Goat Polyclonal to Human BMI1 / PCGF4
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Immunohistochemistry

Immunohistochemistry
Anti-BMI-1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B346 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Anti-Bmi1 Antibody - Immunofluorescence Microscopy. Immunofluorescence using affinity purified goat anti-Bmi1 shows nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3 M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4°C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).

Western Blot

Western Blot
Anti-Bmi1 Antibody - Western Blot. Western blot of Affinity Purified anti-Bmi1 antibody shows detection of a band ~37 kD corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4C with the primary antibody diluted to 1:1000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 4/24/2014
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