Target
Human Betaglycan / TGFBR3
Synonyms
TGFBR3 | Betaglycan | BGCAN | TGF-beta receptor type 3 | TGF-beta receptor type III | TGFR-3 | Betaglycan proteoglycan | Tgf-beta receptors type iii
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
NS0-derived rhTGFb sRIII extracellular domain.
Epitope
Extracellular Domain
Specificity
Recognizes human TGFb RIII. Based on direct ELISA and Western Blot results, this antibody shows less than 1% cross-reactivity with rhTGFb RII.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Flow Cytometry (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human TGF- RIII. The detection limit for rhTGF- RIII is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. Tested in flow cytometry using HeLa cells. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human TGF- RIII. The detection limit for rhTGF- RIII is approximately 0.16 ng/well.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
1.25 ml sterile PBS
Storage
Reconstitute with sterile PBS and store at -20°C. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Betaglycan / TGFBR3