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Anti-BAG3 / BAG-3 Antibody (C-Terminus) LS-C148218

Ordering

Wt. Vol. Conc. Price
- 50 µl - $335

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

BAG3 / BAG-3 Antibody LS-C148218 Images

[click image for a larger image and more details]
Immunohistochemistry
Frozen mouse muscle tissue section stained for BAG-3 expression using LS-C148218 at 1:2000.
Immunohistochemistry
Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections using...
Western Blot
Western blot of BAG-3 using LS-C148218 at 1:2000.
 

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Rabbit Polyclonal to Human BAG3 / BAG-3
Human, Mouse
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated

Details

Human BAG3 / BAG-3
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unpurified serum

Applications

IHC - Paraffin (1:1000 - 1:5000), IHC - Frozen, Western blot (1:1000 - 1:2000), Immunoprecipitation (1:50 - 1:200)

Specificity and Use

BAG3 / BAG-3 antibody was raised against a recombinant protein fragment corresponding to the C-terminal 196 amino acids of human BAG-3.
C-Terminus
Human BAG3

Packaging

Antiserum, 0.05% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BAG3 / BAG-3

O95817 NM_004281 NP_004272.2

BAG3 Antibody, Bcl-2-associated athanogene 3 Antibody, Bcl-2-binding protein Bis Antibody, BCL2-binding athanogene 3 Antibody, BIS Antibody, BAG-3 Antibody, CAIR-1 Antibody, Docking protein cair1 Antibody, BCL2-associated athanogene 3 Antibody, Docking protein CAIR-1 Antibody

This protein has a number of names including Bis, CAIR-1, and BAG-3 and belongs to a family of proteins that share the BAG domain. BAG-3 binds to HSP-70 and inhibits Hsp-mediated proteasomal degradation by virtue of its C-terminal BAG domain that interacts with the ATP binding site of Hsp-70. This protein has also been shown to interact with Bcl-2 and by it self exerts weak anti-apoptotic activity but is synergistic with Bcl-2 in preventing Bax-induced and Fas-mediated apoptosis.

Immunohistochemistry

Immunohistochemistry
Frozen mouse muscle tissue section stained for BAG-3 expression using LS-C148218 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.

Immunohistochemistry

Immunohistochemistry
Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections using LS-C148218 at 1:2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-Eosin counterstain.

Western Blot

Western Blot
Western blot of BAG-3 using LS-C148218 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.
 

Requested From: United States
Date Requested: 9/18/2014

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