LSBio The Immunohistochemistry Antibody Company
  • Home
  • Antibodies
    • Primary Antibodies
    • Secondary Antibodies
    • IHC-plus Antibodies
    • Isotype Control Antibodies
  • ELISA Kits
    • All Kits
    • Sandwich ELISA Kits
    • EIA Kits
    • Cell-Based ELISA Kits
    • Dna-Binding ELISA Kits
  • Services
    • Immunohistochemistry Studies
    • Tissue Cross-Reactivity Screening
  • Distributors
  • Resources
    • About our Tissue Bank
    • Customer Support
    • How to Buy
    • IHC-plus Protocol
    • LSBio Media
    • Publications
    • Antibody Reports
  • Company
    • About LifeSpan
    • Press Releases
    • Holiday Schedule
    • Contact Us
  • View Cart

Anti-BAG3 / BAG-3 Antibody (aa537-548) IHC-plus™ LS-B10292

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

BAG3 / BAG-3 Antibody LS-B10292 Images

[click image for a larger image and more details]
Immunohistochemistry
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot
BAG3 antibody (0.1 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer).

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human BAG3 / BAG-3
Human, Chimpanzee
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human BAG3 / BAG-3
Goat
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.1 - 0.3 µg/ml), ELISA (1:16000)

Specificity and Use

BAG3 / BAG-3 antibody was raised against synthetic peptide C-KNAGNAEDPHTE from an internal region of human BAG3 / BAG-3 (NP_004272.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon, Monkey (92%).
aa537-548
Human BAG3 / BAG-3
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 70kD band observed in Human Pancreas lysates (calculated MW of 61.6kD according to NP_004272.2). Recommended concentration: 0.1-0.3 ug/ml.

Packaging

Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About BAG3 / BAG-3

O95817 NM_004281 NP_004272.2

BAG3 Antibody, Bcl-2-associated athanogene 3 Antibody, Bcl-2-binding protein Bis Antibody, BCL2-binding athanogene 3 Antibody, BIS Antibody, BAG-3 Antibody, CAIR-1 Antibody, Docking protein cair1 Antibody, BCL2-associated athanogene 3 Antibody, Docking protein CAIR-1 Antibody

This protein has a number of names including Bis, CAIR-1, and BAG-3 and belongs to a family of proteins that share the BAG domain. BAG-3 binds to HSP-70 and inhibits Hsp-mediated proteasomal degradation by virtue of its C-terminal BAG domain that interacts with the ATP binding site of Hsp-70. This protein has also been shown to interact with Bcl-2 and by it self exerts weak anti-apoptotic activity but is synergistic with Bcl-2 in preventing Bax-induced and Fas-mediated apoptosis.

Immunohistochemistry

Immunohistochemistry
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western Blot

Western Blot
BAG3 antibody (0.1 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 9/21/2014

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2014 LifeSpan BioSciences, Inc. All Rights Reserved