Human, Mouse, Dog (tested or 100% immunogen sequence identity)
Western blot (0.2 - 1 µg/ml)
Specificity and Use
ATP5F1 antibody was raised against synthetic peptide from internal region of human ATP5F1 (NP_001679.2, P24539). Percent identity by BLAST analysis: Human, Mouse, Dog (100%); Pig (92%).
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.
Lyophilized from PBS with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.