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Anti-APRT Antibody (N-Terminus) IHC-plus™ LS-B10419

Ordering

Wt. Vol. Conc. Price
50 µg - - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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APRT Antibody LS-B10419 Images

[click image for a larger image and more details]
Immunohistochemistry
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot
APRT antibody Western blot of 293T cell lysate.

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Rabbit Polyclonal (IgG) to Human APRT
Human, Chimpanzee, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated

Details

Human APRT
Rabbit
Human, Chimpanzee, Rat, Dog (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.2 - 1 µg/ml)

Specificity and Use

APRT antibody was raised against synthetic peptide from N-Terminus of human APRT (P07741, NP_000476). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon, Monkey, Hamster, Guinea pig (85%); Elephant (84%); Rat (76%).
LS-E21365 - Lyophilized - 100 µg - $145.00
N-Terminus
Human APRT
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About APRT

P07741 NM_000485 NP_000476.1

APRT Antibody, AMP Antibody, AMP diphosphorylase Antibody, APRTD Antibody, AMP pyrophosphorylase Antibody, Transphosphoribosidase Antibody

The adenine phosphoribosyltransferase is an enzyme involved in the purine salvage pathway. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

Immunohistochemistry

Immunohistochemistry
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western Blot

Western Blot
APRT antibody Western blot of 293T cell lysate.

Requested From: United States
Date Requested: 9/20/2014

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