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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2970-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human APP / Beta Amyloid Precursor Antibody (clone J4H9, aa18‑289, IHC, WB) LS‑B2970
IHC‑plus™ Monoclonal Mouse anti‑Human APP / Beta Amyloid Precursor Antibody (clone J4H9, aa18‑289, IHC, WB) LS‑B2970
Note: This antibody replaces LS-C93706
Antibody:
APP / Beta Amyloid Precursor Mouse anti-Human Monoclonal (aa18-289) (J4H9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
APP / Beta Amyloid Precursor Mouse anti-Human Monoclonal (aa18-289) (J4H9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
Beta Amyloid Precursor antibody LS-B2970 is an unconjugated mouse monoclonal antibody to Beta Amyloid Precursor (APP) (aa18-289) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human APP / Beta Amyloid Precursor
Synonyms
APP | ABPP | A4 | ABETA | AD1 | Alzheimer disease | Amyloid beta | Beta-amyloid peptide | APPI | Beta amyloid | CVAP | AAA | PN2 | PreA4 | Amyloid beta A4 protein | Peptidase nexin-II | CTFgamma | PN-II | Protease nexin-II
Host
Mouse
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Clone
J4H9
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human APP (18-289aa) purified from E. coli.
Epitope
aa18-289
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:500 - 1:1000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2970 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2970 was determined to be 2.5 ug/ml.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Storage
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About APP / Beta Amyloid Precursor
Validation
Anti-APP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-APP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ APP / Beta Amyloid Precursor Antibody (aa18-289, clone J4H9) for IHC, WB/Western, ELISA LS-B2970 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat, Primate
Applications:
Western blot, ELISA
Reactivity:
Rat
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
