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Antibodies > APG5 / ATG5 > LS-C156406
 
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Catalog Number Size Price
LS-C156406-400 400 µl $393 
APG5 / ATG5 Antibody - ATG5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
APG5 / ATG5 Antibody - Fluorescent image of U251 cells stained with ATG5 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG5 / ATG5 Antibody - ATG5 Antibody western blot of uterus tumor cell line lysates (35 ug/lane). The ATG5 antibody detected the ATG5 protein (arrow).
APG5 / ATG5 Antibody - Western blot of lysate from K562 cell line, using APG5 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
APG5 / ATG5 Antibody - Western blot of APG5 (arrow) using rabbit polyclonal APG5 Antibody. 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5 gene.
APG5 / ATG5 Antibody - ATG5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
APG5 / ATG5 Antibody - Fluorescent image of U251 cells stained with ATG5 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG5 / ATG5 Antibody - ATG5 Antibody western blot of uterus tumor cell line lysates (35 ug/lane). The ATG5 antibody detected the ATG5 protein (arrow).
APG5 / ATG5 Antibody - Western blot of lysate from K562 cell line, using APG5 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
APG5 / ATG5 Antibody - Western blot of APG5 (arrow) using rabbit polyclonal APG5 Antibody. 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5 gene.
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Polyclonal Rabbit anti‑Human APG5 / ATG5 Antibody (IHC, IF, WB) LS‑C156406

Polyclonal Rabbit anti‑Human APG5 / ATG5 Antibody (IHC, IF, WB) LS‑C156406

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
APG5 / ATG5 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C156406-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APG5 / ATG5 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ATG5 antibody LS-C156406 is an unconjugated rabbit polyclonal antibody to human ATG5 (APG5). Validated for IF, IHC and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human APG5 / ATG5
Synonyms
ATG5 | APG5 | APG5-LIKE | APG5L | Apoptosis specific protein | Apoptosis-specific protein | Autophagy related 5 | HAPG5 | ASP | Autophagy protein 5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG5 / ATG5
Q9H1Y0 NM_004849 NP_004840.1

LSBio Ratings

APG5 / ATG5 Antibody for IHC, IF/Immunofluorescence, WB/Western LS-C156406 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

Autophagy induced by deficiency of sphingosine-1-phosphate phosphohydrolase 1 is switched to apoptosis by calpain-mediated autophagy-related gene 5 (Atg5) cleavage. Lpine S, Allegood JC, Edmonds Y, Milstien S, Spiegel S. The Journal of biological chemistry. 2011 286:44380-90.
PubMed: 22052905 PMC: PMC3247960
Submit a Publication for LS-C156406

Customer Reviews (0)

Submit a review for LS-C156406

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Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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