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Anti-ANXA1 / Annexin A1 Antibody (C-Terminus) IHC-plus™ LS-B3028

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

ANXA1 / Annexin A1 Antibody LS-B3028 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-Annexin A1 antibody IHC of human placenta.
Immunohistochemistry
Anti-Annexin A1 antibody IHC of human prostate.
Western Blot
Antibody (0.5 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer).
 

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Goat Polyclonal to Human ANXA1 / Annexin A1
Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ANXA1 / Annexin A1
Goat
Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (4 µg/ml), Western blot (0.5 - 1.5 µg/ml), ELISA (1:32000)

Specificity and Use

ANXA1 / Annexin A1 antibody was raised against synthetic peptide CQAILDETKGDYEK from the C-terminus of human ANXA1 / Annexin A1 (NP_000691.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Guinea pig (100%); Platypus (93%); Lizard (86%).
LS-E26990 - Lyophilized - 100 µg - $145.00
C-Terminus
Human ANXA1 / Annexin A1.
Immunohistochemistry: LS-B3028 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3028 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kD according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al, Cancer Res. 2000 Nov15;60(22):6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

Publications (1)

1
Exposure to chemical cocktails before or after conception – The effect of timing on ovarian development. Bellingham M, Amezaga MR, Mandon-Pepin B, Speers CJ, Kyle CE, Evans NP, Sharpe RM, Cotinot C, Rhind SM, Fowler PA. Molecular and cellular endocrinology. 2013 Aug;376:156-72. (WB; Sheep) [Full Text Article] [PubMed:23791816]

About ANXA1 / Annexin A1

P04083 NM_000700 NP_000691.1

ANXA1 Antibody, Annexin A1 Antibody, Annexin I Antibody, Annexin-1 Antibody, ANX1 Antibody, Calpactin II Antibody, Chromobindin-9 Antibody, Lipocortin I Antibody, Annexin I (lipocortin I) Antibody, Calpactin-2 Antibody, LPC1 Antibody

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35000 to 40000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kD, with phospholipase A2 inhibitory activity.

Immunohistochemistry

Immunohistochemistry
Anti-Annexin A1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3028 concentration 4 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Annexin A1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3028 concentration 4 ug/ml.

Western Blot

Western Blot
Antibody (0.5 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 10/23/2014

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