Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
IHC - Paraffin (4 µg/ml), Western blot (0.5 - 1.5 µg/ml), ELISA (1:32000)
Specificity and Use
ANXA1 / Annexin A1 antibody was raised against synthetic peptide CQAILDETKGDYEK from the C-terminus of human ANXA1 / Annexin A1 (NP_000691.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Guinea pig (100%); Platypus (93%); Lizard (86%).
Human ANXA1 / Annexin A1.
Immunohistochemistry: LS-B3028 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3028 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kD according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al, Cancer Res. 2000 Nov15;60(22):6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 ug/ml.
Exposure to chemical cocktails before or after conception – The effect of timing on ovarian development. Bellingham M, Amezaga MR, Mandon-Pepin B, Speers CJ, Kyle CE, Evans NP, Sharpe RM, Cotinot C, Rhind SM, Fowler PA. Molecular and cellular endocrinology. 2013 Aug;376:156-72. (WB; Sheep)[Full Text Article]
ANXA1 Antibody, Annexin A1 Antibody, Annexin I Antibody, Annexin-1 Antibody, ANX1 Antibody, Calpactin II Antibody, Chromobindin-9 Antibody, Lipocortin I Antibody, Annexin I (lipocortin I) Antibody, Calpactin-2 Antibody, LPC1 Antibody
Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35000 to 40000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kD, with phospholipase A2 inhibitory activity.