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Catalog Number Size Price
LS-B10714-200 200 µl (0.5 mg/ml) $460 
ALOX12 / 12 Lipoxygenase Antibody - Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
ALOX12 / 12 Lipoxygenase Antibody - Immunofluorescence of A549 cells, using ALOX12 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
ALOX12 / 12 Lipoxygenase Antibody - Western blot of ALOX12 Antibody in K562 cell line lysates (35 ug/lane). ALOX12 (arrow) was detected using the purified antibody.
ALOX12 / 12 Lipoxygenase Antibody - ALOX12 Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ALOX12 / 12 Lipoxygenase Antibody - Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
ALOX12 / 12 Lipoxygenase Antibody - Immunofluorescence of A549 cells, using ALOX12 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
ALOX12 / 12 Lipoxygenase Antibody - Western blot of ALOX12 Antibody in K562 cell line lysates (35 ug/lane). ALOX12 (arrow) was detected using the purified antibody.
ALOX12 / 12 Lipoxygenase Antibody - ALOX12 Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC‑plus™ Polyclonal Rabbit anti‑Human ALOX12 / 12 Lipoxygenase Antibody (aa618‑650, IHC, IF, WB) LS‑B10714

IHC‑plus™ Polyclonal Rabbit anti‑Human ALOX12 / 12 Lipoxygenase Antibody (aa618‑650, IHC, IF, WB) LS‑B10714

Note: This antibody replaces LS-C156288
Antibody:
ALOX12 / 12 Lipoxygenase Rabbit anti-Human Polyclonal (aa618-650) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B10714-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ALOX12 / 12 Lipoxygenase Rabbit anti-Human Polyclonal (aa618-650) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
12 Lipoxygenase antibody LS-B10714 is an unconjugated rabbit polyclonal antibody to human 12 Lipoxygenase (ALOX12) (aa618-650). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ALOX12 / 12 Lipoxygenase
Synonyms
ALOX12 | 12S-lipoxygenase | 12-lipoxygenase | 12S-LOX | 12(S)-lipoxygenase | 12-LOX | 12 Lipoxygenase | Arachidonate 12-lipoxygenase | LOG12 | Platelet 12-LOX | Platelet-type 12-lipoxygenase | Platelet-type lipoxygenase 12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa618-650
Specificity
This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12.
Applications
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALOX12 / 12 Lipoxygenase
P18054 NM_000697 NP_000688.2

Validation

ALOX12 / 12 Lipoxygenase Antibody - Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

LSBio Ratings

IHC-plus™ ALOX12 / 12 Lipoxygenase Antibody (aa618-650) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B10714 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/17/2024