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Anti-AGT / Angiotensinogen Antibody (aa1-476) LS-C185567

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $335

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206-374-1102
866-206-6909
Orders@LSBio.com
 

AGT / Angiotensinogen Antibody LS-C185567 Images

[click image for a larger image and more details]
Immunohistochemistry
IHC of paraffin-embedded Hepatocellular carcinoma Huh7 xenograft using AGT antibody at 1:100...
ICC
Immunofluorescence of methanol-fixed HepG2 using Angiotensinogen antibody at 1:200 dilution.
Western Blot
Sample (30 ug whole cell lysate).
 

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Rabbit Polyclonal (IgG) to Human AGT / Angiotensinogen
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human AGT / Angiotensinogen
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1:100 - 1:1000), ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)

Specificity and Use

AGT / Angiotensinogen antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 476 of Angiotensinogen (SwissProt P01019).
aa1-476
Human Angiotensin II
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About AGT / Angiotensinogen

P01019 NM_000029 NP_000020.1

AGT Antibody, Angiotensin I Antibody, ANHU Antibody, Angiotensinogen Antibody, Pre-angiotensinogen Antibody, Angiotensin II Antibody, SERPINA8 Antibody, Serpin A8 Antibody

The human AGT gene product, pre-angiotensinogen, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Human pre-angiotensinogen is encoded by two mRNAs that differ only in the length of the 3'-untranslated region due to postulated use of two polyadenylation sites.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Hepatocellular carcinoma Huh7 xenograft using AGT antibody at 1:100 dilution.

ICC

ICC
Immunofluorescence of methanol-fixed HepG2 using Angiotensinogen antibody at 1:200 dilution.

Western Blot

Western Blot
Sample (30 ug whole cell lysate). A: Hep G2 . 10% SDS PAGE. Angiotensin II antibody diluted at 1:1000
 

Requested From: United States
Date Requested: 10/24/2014

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