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Anti-ACAT1 Antibody (clone AT2C5) IHC-plus™ LS-B8975

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395

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ACAT1 Antibody LS-B8975 Images

[click image for a larger image and more details]
Immunohistochemistry
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot
Western Blot: The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane...

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Mouse Monoclonal [clone AT2C5] (IgG1,k) to Human ACAT1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ACAT1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [AT2C5]
Protein A purified

Applications

IHC - Paraffin (10 µg/ml), Western blot (1:1000), ELISA

Specificity and Use

ACAT1 antibody was raised against recombinant human ACAT1 (34-427aa) purified from E. coli.
Human ACAT1

Packaging

PBS, pH 7.4, 0.1% sodium azide
Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.
For research use only.

About ACAT1

P24752 NM_000019 NP_000010.1

ACAT1 Antibody, ACAT Antibody, Acetoacetyl-CoA thiolase Antibody, Acetyl-CoA acetyltransferase 1 Antibody, MAT Antibody, T2 Antibody, THIL Antibody

The ACAT1 mRNA encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The ACAT gene spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Immunohistochemistry

Immunohistochemistry
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western Blot

Western Blot
Western Blot: The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: United States
Date Requested: 10/24/2014

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