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See All 48 Nestin Antibodies

Ordering
Wt. Vol. Conc. Price
50 µg - 1 mg/ml $385

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206-374-1102
866-206-6909
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Nestin Antibody LS-B656 Images

Immunohistochemistry
Anti-Nestin antibody IHC staining of mouse kidney.
Immunohistochemistry
Anti-Nestin antibody IHC staining of human kidney.
Western Blot
Anti-Nestin Antibody - Western Blot.
Western Blot
Anti-Nestin Antibody - Western Blot.

Anti-Nestin Antibody LS-B656 IHC-plus™

Rabbit anti-Human Polyclonal, aa1484-1500

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Details

Applications
Human Nestin
Rabbit
Human
Polyclonal
Immunoaffinity Chromatography
IHC-P (5 µg/ml)
Western blot (1:500 - 1:3000)
IF
ELISA (1:20000 - 1:80000)
Optimal dilution to be determined by the researcher.

Specificity and Use

Nestin antibody was raised against recombinant human NES / Nestin.
aa1484-1500
Amino acids 1484-1500 of human Nestin protein.
Immunohistochemistry: LS-B656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B656 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About Nestin

P48681 X65964 CAA46780.1

NES Antibody, Nestin Antibody, Nbla00170 Antibody

Nestin (NES) is an intermediate filament protein. Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
(More About Nestin)

Nestin Antibodies (48)

Target Host Clonality Format Epitope Reactivity Applications
Nestin Rabbit Polyclonal Human ELISA, IF, IHC-P, WB
Nestin Antibody LS-B656 Rabbit Polyclonal thumb(5)
50 µg - 385.00
Applications: IHC-P, IF, WB... Epitope: aa1484-1500  
Reactivity: Hu Format:  
Nestin Chicken Polyclonal Human, Mouse ICC, IHC-P, WB
Nestin Antibody LS-B5224 Chicken Polyclonal thumb(1)
100 µl - 385.00
Applications: IHC-P, ICC, WB Epitope:  
Reactivity: Hu, Mu Format:  
Nestin Mouse Monoclonal Human IF, IHC-P, WB
Nestin Antibody [10C2] LS-B51 Mouse Monoclonal thumb(1)
100 µl - 385.00
Applications: IHC-P, IF, WB Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human IHC-P, WB
Nestin Antibody LS-B678 Rabbit Polyclonal thumb(2)
50 µl - 385.00
Applications: IHC-P, WB Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human ICC, IHC-Fr, IHC-P, WB
Nestin Antibody [10C2] LS-B7181 Mouse Monoclonal thumb(3)
50 µl - 385.00
Applications: IHC-Fr, IHC-P, ICC... Epitope:  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human IHC-Fr, IHC-P, WB
Nestin Antibody [10C2] LS-B7877 Mouse Monoclonal thumb(2)
50 µg - 385.00
Applications: IHC-Fr, IHC-P, WB Epitope:  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human ICC, IF, IHC-Fr, IHC-P, WB
Nestin Antibody LS-B2777 Mouse Monoclonal thumb(1)
50 µl - 385.00
Applications: IHC-Fr, IHC-P, ICC... Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Dog, Human IHC-P, WB
Nestin Antibody LS-B5875 Rabbit Polyclonal thumb(2)
50 µg - 385.00
Applications: IHC-P, WB Epitope: Internal  
Reactivity: Do, Hu Format:  
Nestin Mouse Monoclonal Human Flo, ICC, IHC-P, IP, WB
Nestin Antibody [2C1.3A11] LS-B2851 Mouse Monoclonal thumb(1)
50 µl - 385.00
Applications: IHC-P, ICC, WB... Epitope:  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Mouse, Rat IHC-Fr, WB
Nestin Antibody [Rat-401, 4D4] LS-C42054 Mouse Monoclonal
100 µg - 490.00
Applications: IHC-Fr, WB Epitope:  
Reactivity: Mu, Rt Format:  
Nestin Rabbit Polyclonal Human, Mouse, Rat WB
Nestin Antibody LS-C137454 Rabbit Polyclonal
100 µl - 260.00
Applications: WB Epitope:  
Reactivity: Hu, Mu, Rt Format:  
Nestin Rabbit Polyclonal Human Flo, IHC-P, WB
Nestin Antibody LS-C97685 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IHC-P, WB, Flo Epitope: Ser1409  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Chimpanzee, Human IHC-P, WB
Nestin Antibody LS-C148621 Rabbit Polyclonal thumb(2)
100 µg - 330.00
Applications: IHC-P, WB Epitope: aa1450-1500  
Reactivity: Ch, Hu Format:  
Nestin Mouse Monoclonal Human ICC, IF, IHC, IHC-Fr, IHC-P, WB
Nestin Antibody [10C2] LS-C142111 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, IHC-Fr... Epitope: aa1464-1614  
Reactivity: Hu Format: Biotin  
Nestin Mouse Monoclonal Human ICC, IF, IHC, IHC-Fr, IHC-P, WB
Nestin Antibody [10C2] LS-C142112 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, IHC-Fr... Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human ICC, IF, IHC, IHC-Fr, IHC-P, WB
Nestin Antibody [10C2] LS-C142113 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, IHC-Fr... Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human ICC, IF, IHC, IHC-Fr, IHC-P, WB
Nestin Antibody [10C2] LS-C142114 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, IHC-Fr... Epitope: aa1464-1614  
Reactivity: Hu Format: HRP  
Nestin Mouse Monoclonal Human ICC, IF, IHC, IHC-Fr, IHC-P, WB
Nestin Antibody [10C2] LS-C142115 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, IHC-Fr... Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human IHC-Fr, IHC-P, WB
Nestin Antibody LS-C45602 Mouse Monoclonal
100 µg - 550.00
Applications: IHC-Fr, IHC-P, WB Epitope:  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human IHC-Fr, IHC-P, WB
Nestin Antibody LS-C45603 Rabbit Polyclonal
50 µl - 550.00
Applications: IHC-Fr, IHC-P, WB Epitope:  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human ELISA, ICC, IHC-Fr, IHC-P, WB
Nestin Antibody LS-C25742 Mouse Monoclonal
50 µl - 435.00
Applications: IHC-Fr, IHC-P, ICC... Epitope:  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human IHC-Fr, IHC-P, WB
Nestin Antibody LS-C122204 Rabbit Polyclonal
50 µl - 680.00
Applications: IHC-Fr, IHC-P, WB Epitope:  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human IHC-Fr, IHC-P, WB
Nestin Antibody LS-C122205 Mouse Monoclonal
100 µg - 680.00
Applications: IHC-Fr, IHC-P, WB Epitope:  
Reactivity: Hu Format:  
Nestin Goat Polyclonal Rat ICC, IHC-P, WB
Nestin Antibody LS-C150302 Goat Polyclonal thumb(3)
100 µg - 395.00
Applications: IHC-P, ICC, WB Epitope:  
Reactivity: Rt Format:  
Nestin Mouse Monoclonal Human Flo, IHC-P, WB
Nestin Antibody [10C2] LS-C179723 Mouse Monoclonal thumb(1)
100 µg - 325.00
Applications: IHC-P, WB, Flo Epitope:  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human, Mouse ELISA, IF, IHC, WB
Nestin Antibody LS-C146886 Rabbit Polyclonal
100 µg - 445.00
Applications: IHC, IF, WB... Epitope:  
Reactivity: Hu, Mu Format:  
Nestin Chicken Polyclonal Mouse, Rat ICC, IHC, WB
Nestin Antibody LS-C149126 Chicken Polyclonal
300 µg - 455.00
Applications: IHC, ICC, WB Epitope:  
Reactivity: Mu, Rt Format:  
Nestin Rabbit Polyclonal Human IHC-P
Nestin Antibody LS-C108411 Rabbit Polyclonal
0.1 ml - 175.00
Applications: IHC-P Epitope:  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human IHC-P
Nestin Antibody LS-C108412 Rabbit Polyclonal
1 ml - 435.00
Applications: IHC-P Epitope:  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Mouse, Rat ICC, IHC-Fr, WB
Nestin Antibody LS-C25743 Mouse Monoclonal
100 µg - 440.00
Applications: IHC-Fr, ICC, WB Epitope:  
Reactivity: Mu, Rt Format:  
Nestin Chicken Polyclonal Mouse ICC, IHC, WB
Nestin Antibody LS-C124240 Chicken Polyclonal
1000 µl - 525.00
Applications: IHC, ICC, WB Epitope:  
Reactivity: Mu Format:  
Nestin Chicken Polyclonal Bovine, Human IHC, WB
Nestin Antibody LS-C73311 Chicken Polyclonal
250 µl - 495.00
Applications: IHC, WB Epitope:  
Reactivity: Bo, Hu Format:  
Nestin Rabbit Polyclonal Human ELISA, IHC
Nestin Antibody LS-C124238 Rabbit Polyclonal
100 µg - 455.00
Applications: IHC, ELISA Epitope: N-Terminus  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human Flo, IF, WB
Nestin Antibody LS-C164830 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IF, WB, Flo Epitope:  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human, Mouse, Rat ICC, WB
Nestin Antibody [4D11] LS-C180217 Mouse Monoclonal thumb(2)
100 µl - 335.00
Applications: ICC, WB Epitope:  
Reactivity: Hu, Mu, Rt Format:  
Nestin Mouse Monoclonal Mouse, Rat ICC, WB
Nestin Antibody [307501] LS-C150275 Mouse Monoclonal thumb(1)
100 µg - 320.00
Applications: ICC, WB Epitope:  
Reactivity: Mu, Rt Format:  
Nestin Mouse Monoclonal Mouse IF
Nestin Antibody [Rat-401 (4D4)] LS-C106594 Mouse Monoclonal thumb(1)
25 µg - 215.00
100 µg - 320.00
Applications: IF Epitope:  
Reactivity: Mu Format:  
Nestin Rabbit Polyclonal Human Flo, WB
Nestin Antibody LS-C146896 Rabbit Polyclonal
100 µg - 445.00
Applications: WB, Flo Epitope:  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human WB
Nestin Antibody LS-C142106 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa1464-1614  
Reactivity: Hu Format: Biotin  
Nestin Rabbit Polyclonal Human WB
Nestin Antibody LS-C142107 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human WB
Nestin Antibody LS-C142108 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human WB
Nestin Antibody LS-C142109 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa1464-1614  
Reactivity: Hu Format: HRP  
Nestin Rabbit Polyclonal Human WB
Nestin Antibody LS-C142110 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human Flo, ICC
Nestin Antibody LS-C25741 Mouse Monoclonal
100 µg - 455.00
Applications: ICC, Flo Epitope: aa618-1618  
Reactivity: Hu Format:  
Nestin Mouse Monoclonal Human ELISA, ICC
Nestin Antibody LS-C73312 Mouse Monoclonal
50 µg - 535.00
Applications: ICC, ELISA Epitope: aa618-1618  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human WB
Nestin Antibody LS-C73313 Rabbit Polyclonal
100 µl - 445.00
Applications: WB Epitope: aa1464-1614  
Reactivity: Hu Format:  
Nestin Rabbit Polyclonal Human ELISA
Nestin Antibody LS-C25740 Rabbit Polyclonal
100 µg - 480.00
Applications: ELISA Epitope:  
Reactivity: Hu Format:  
Nestin Goat Polyclonal Rat WB
Nestin Antibody LS-C131008 Goat Polyclonal
50 µg - 580.00
Applications: WB Epitope: aa544-776  
Reactivity: Rt Format: Biotin  

Immunohistochemistry

Immunohistochemistry
Anti-Nestin antibody IHC staining of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B656 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Nestin antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B656 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-Nestin Antibody - Western Blot. Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to human Nestin (arrowhead). Undifferentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 1), or differentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 2) were separated by SDS-PAGE using 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2000 overnight at 4°C. The membrane was then washed and reacted with a 1:10000 dilution of peroxidase conjugated affinity purified Gt-a-Rabbit IgG [H&L] MX (LS-C60884) for 45 min at room temperature. Image was captured using film. Other detection systems will yield similar results. Image courtesy of Prof. F. H. Gage of the Salk Institute, San Diego, CA.

Western Blot

Western Blot
Anti-Nestin Antibody - Western Blot. Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to mouse Nestin (arrowhead). Approximately 30 g of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2000 overnight at 4°C. The membrane was then washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Ddyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Requested From: United States
Date Requested: 6/17/2013
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