|
The Immunohistochemistry Antibody Company
|
|
|
Ordering |
|
|
LSBio (Direct) |
|
|
CD223 / LAG-3 Antibody LS-B2237 Images
Anti-LAG-3 antibody IHC staining of human tonsil.
|
Anti-CD223 / LAG-3 Antibody LS-B2237 IHC-plus™
Mouse anti-Human Monoclonal, N-Terminus, Clone 17B4, FITC
100% Guaranteed
|
Details |
Applications |
|||||||||||||
|
|
Specificity and Use
| CD223 / LAG-3 antibody was raised against synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3). Percent identity by BLAST analysis: Human (100%); Gorilla (93%); Gibbon (90%); Monkey (83%). | |
| N-Terminus | |
| Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of human LAG-3. | |
| Immunohistochemistry: LS-B2237 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2237 was determined to be 10 ug/ml. |
Packaging
| PBS containing 50mM Tris-HCl, 1% BSA, 0.02% sodium azide. | |
| Store at 4°C. Product is photosensitive and should be protected from light. | |
| For research use only. |
About CD223 / LAG-3
P18627
NM_002286
LAG3 Antibody, CD223 antigen Antibody, LAG-3 Antibody, Protein FDC Antibody, CD223 Antibody, FDC Antibody, Lymphocyte-activation gene 3 Antibody
Lymphocyte-Activation Protein 3 (LAG3) belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons.
PDF Spec Sheet
Technical HelpCD223 / LAG-3 Antibodies (12)
| Target | Host | Clonality | Format | Epitope | Reactivity | Applications | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD223 / LAG-3 | Mouse | Monoclonal | Human | Flo, IHC-P |
|
||||||||||||
| CD223 / LAG-3 | Mouse | Monoclonal | Human | ICC, WB |
|
||||||||||||
| CD223 / LAG-3 | Mouse | Monoclonal | Human | ELISA |
|
||||||||||||
| CD223 / LAG-3 | Rabbit | Polyclonal | Human | IHC, WB |
|
||||||||||||
| CD223 / LAG-3 | Mouse | Monoclonal | Human | ELISA, ICC, IHC-Fr, IP, WB |
|
||||||||||||
| CD223 / LAG-3 | Rabbit | Polyclonal | Human | WB |
|
||||||||||||
| CD223 / LAG-3 | Rat | Monoclonal | Mouse | Flo, Func |
|
||||||||||||
| CD223 / LAG-3 | Rat | Monoclonal | Mouse | Flo |
|
||||||||||||
| CD223 / LAG-3 | Rat | Monoclonal | Mouse | Flo |
|
||||||||||||
| CD223 / LAG-3 | Rat | Monoclonal | Mouse | Flo, Func |
|
||||||||||||
| CD223 / LAG-3 | Goat | Polyclonal | Human | Flo |
|
||||||||||||
| CD223 / LAG-3 | Goat | Polyclonal | Mouse | Flo |
|
|
Requested From: United States
Date Requested: 5/23/2013
Date Requested: 5/23/2013