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by Dr. Glenna Burmer April 12, 2011
LSBio (LifeSpan BioSciences) offers a catalog of 92,700 antibodies that have been tested for use in a
variety of research applications, including immunohistochemistry, ELISA, Western blot, and flow cytometry. Antibodies can often be used in multiple assays, but they do
not perform equally well in all assays. This is particularly true for immunohistochemistry (IHC). Many antibodies that perform well in other assays do not work well in
IHC against formalin-fixed paraffin-embedded tissues (FFPE-IHC). In immunohistochemistry, antibodies may produce no signal, produce a weak signal, show nonspecific
background staining that interferes with analysis, or show false positive signals. LSBio's goal in immunohistochemistry validation is to identify for our customers
those antibodies that perform well in FFPE-IHC. Out of the 92,700 antibodies in the LSBio catalog, 35,000 have been tested and received validation for use in IHC by
LSBio or through collaborators or suppliers. Of these, 5,500 antibodies have been extensively tested in our Seattle laboratory and awarded IHC-plus™ brand validation.
IHC-plus™ antibodies have been identified as the best reagents for use in FFPE-IHC. [More]
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