LSBio is the world's largest supplier of Immunohistochemistry (IHC) antibodies with more than 27,000 having been tested and approved for use in IHC. More than 4,500 of these antibodies have been further validated specifically for use under LSBio's standardized IHC conditions against formalin-fixed paraffin-embedded human tissues. These are LSBio's premier IHC-plus™ brand antibodies.

Human placenta stained in IHC with SDC1 antibody LS-B3341

Human skin stained in IHC with Vimentin antibody LS-B1942

About our IHC Validation

LSBio's IHC-plus™ antibodies have been tested and identified as being optimal for use in immunohistochemistry (IHC) against formalin-fixed paraffin-embedded (FFPE) human tissues under LSBio's standardized IHC-plus™ protocol.

Each antibody is tested at multiple concentrations on more than 20 normal human tissue types, and when appropriate, multiple normal brain regions and/or cancer types.  LSBio's IHC protocol has been developed over the past 15 years as the most optimal method of immunolabeling FFPE tissues, the most common fixation method used by pathology labs worldwide.  A LifeSpan pathologist, with extensive experience evaluating IHC, analyzes the localization profile of each antibody, identifying positive and negative cell types, signal strength, subcellular and extracellular staining, and staining artifacts.  This information is then compared with all published expression and localization data available for the protein.  This enables LSBio to evaluate how each antibody behaves in IHC, including its specificity to the target protein, its sensitivity of detection, and any non-specific staining characteristics that it may display.  In order to be selected as an IHC-plus™ brand antibody, antibodies must have a close correlation to the published literature, be high affinity, display minimal staining artifacts, and have a high signal-to-noise ratio, such that its specific staining is considerably higher than its level of nonspecific background staining.

Only the best antibodies receive approval and are given the designation as LSBio's premier IHC-plus™ antibodies.

Learn more about LSBio's Immunohistochemistry (IHC) Validation

About our Quality

All antibodies are quality control checked to ensure that they meet LSBio's rigorous standards and each IHC-plus™ antibody is guaranteed to work in immunohistochemistry as outlined on its specification sheet.

About our Support

LSBio's customer support is unparalleled in the antibody industry. Experienced Ph.D. scientists are available to answer questions about antibody specifications, pre-order selection, and post order application trouble shooting.