| Antibody Catalog ID: |
LS-B1733 |
| Antibody Validation: |
This antibody replaces catalog number LS-C2831. It has been validated for use in the following assays: IHC. |
| Antibody Target: |
Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) |
| Synonyms: |
PDK1, PDH kinase 1, PDHK, PDHK1, pdk1, Pyruvate dehydrogenase kinase 1, pyruvate dehydrogenase kinase isoenzyme 1 |
| Family: |
Protein Kinase |
| Subfamily: |
PDK/BCKDK |
| Host: |
PDK1 antibody was produced in Rabbit |
| Antigen Species: |
PDK1 antibody was raised against Human |
| Clonality: |
Polyclonal |
| Antigen Type: |
Synthetic peptide - KLH conjugated |
| Immunogen: |
PDK1 antibody was raised against synthetic peptide |
| Antibody Specificity: |
A synthetic peptide made to a C-Terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118].
|
| Antigen Modification: |
aa350-436
|
| Predicted Species Reactivity: |
Mouse, Rat
|
| Purification: |
Immunoaffinity Chromatography |
| Presentation: |
Tris-glycine buffer, 150 mM sodium chloride. |
| Recommended Storage: |
+4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: |
Immunohistochemistry: LS-B1733 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1733 was determined to be 5 ug/ml. |
| Antibody Uses: |
Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml ), Western blot (WB) (2 µg/ml ) (Optimal dilution to be determined by the researcher) |
| Size: |
50 µl |
| Concentration: |
0.4 mg/ml |
| Antibody Price (USD): |
$350 |
|
|
Immunohistochemistry Image:
|
|
| Adrenal Cortex: Formalin-Fixed Paraffin-Embedded (FFPE) |
| |
|
|
Recommended Secondary Antibodies (1)
|
| Target | Catalog | Type | Form | Epitope | Assay | Reactivity | Size | Price | Buy |
| Rabbit IgG Antibody | LS-D1 | Goat | Biotin | | | Rabbit | 750 µg | $100 |  |
|
|
Related Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibodies (9)
|
| Target | Catalog | Type | Form | Epitope | Assay | Reactivity | Size | Price | Buy |
| Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibody | LS-B689 | Rabbit | | aa300-400 | | Human | 50 µg | $350 | |
| Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibody | LS-B3690 | Rabbit | | | | Human | 50 µg | $350 | |
| Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibody | LS-C26009 | Rabbit IgG | | aa421-436 | | Human | 50 µg | $350 | |
| Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibody | LS-C55254 | Goat | | Internal | | Human | 100 µg | $295 | |
| Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibody | LS-C103496 | Mouse IgG1,k | | | | Human | 50 µl | $255 | |
| Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibody | LS-C98956 | Rabbit | | N-Terminus | | Human | 100 µg | $267 | |
| Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibody | LS-C101357 | Rabbit IgG | | | | Human | 50 µg | $380 | |
| Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibody | LS-C93136 | Rabbit | | C-Terminus | | Human | 100 µl | $445 | |
| Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) Antibody | LS-C93137 | Rabbit | | Internal | | Human | 100 µl | $445 | |
|
|
| Requested From: |
United States |