| Antibody Catalog ID: |
LS-B2042 |
| Antibody Validation: |
This antibody replaces catalog number LS-C795. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: |
5-Ht3a Receptor (HTR3A) |
| Synonyms: |
HTR3A, 5-HT3, 5-HT3 receptor, 5-ht3a, 5-ht3a receptor, 5-HT3R, 5-HT3R-A, 5-hydroxytryptamine receptor 3, 5-hydroxytryptamine receptor 3a, 5HT3R, HTR3, HTR3a, Serotonin-gated ion channel receptor 3a |
| Family: |
Ion channel |
| Subfamily: |
Serotonin 5-HT3 receptor |
| Host: |
HTR3A antibody was produced in Rabbit |
| Antigen Species: |
HTR3A antibody was raised against Rat |
| Clonality: |
Polyclonal |
| Isotype: |
IgG |
| Antigen Type: |
Synthetic peptide - KLH conjugated |
| Immunogen: |
HTR3A antibody was raised against synthetic peptide |
| Antibody Specificity: |
A mixture of synthetic peptides corresponding to amino acids 21-36 and 421-436 of rat 5-HT3 receptor
|
| Antigen Modification: |
aa21-36
|
| Predicted Species Reactivity: |
Human
|
| Purification: |
Protein G Column |
| Presentation: |
PBS, 0.2% gelatin, 0.05% sodium azide. |
| Recommended Storage: |
Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: |
Immunohistochemistry: LS-B2042 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2042 was determined to be 5 ug/ml. |
| Antibody Uses: |
Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml ), Western blot (WB) (1 µg/ml ) (Optimal dilution to be determined by the researcher) |
| Size: |
50 µg |
| Concentration: |
0.5 mg/ml |
| Antibody Price (USD): |
$350 |
|
|
Immunohistochemistry Image:
|
|
| Heart: Formalin-Fixed Paraffin-Embedded (FFPE) |
| |
|
|
Recommended Secondary Antibodies (1)
|
| Target | Catalog | Type | Form | Epitope | Assay | Reactivity | Size | Price | Buy |
| Rabbit IgG Antibody | LS-D1 | Goat | Biotin | | | Rabbit | 750 µg | $100 |  |
|
|
Related 5-Ht3a Receptor (HTR3A) Antibodies (4)
|
| Target | Catalog | Type | Form | Epitope | Assay | Reactivity | Size | Price | Buy |
| 5-Ht3a Receptor (HTR3A) Antibody | LS-B152 | Rabbit | | | | Human | 50 µg | $385 | |
| 5-Ht3a Receptor (HTR3A) Antibody | LS-C91322 | Rabbit IgG | | 1st Extracellular Domain | | Human | 40 µg | $305 | |
| 5-Ht3a Receptor (HTR3A) Antibody | LS-C94880 | Rabbit | | Extracellular Domain | | Mouse | 100 µl | $343 | |
| 5-Ht3a Receptor (HTR3A) Antibody | LS-C55453 | Goat | | Internal | | Human | 100 µg | $295 | |
|
|
Antibody Summary:
Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems. Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455 amino acids. The human subunit exhibits 85% and 84% amino acid sequence identity to the mouse and rat receptors, respectively. Anti-5-HT3R polyclonal antibody shows immunoreactivity in the forebrain (olfactory regions, hippocampal formation and amygdala), sensory and motor nuclei of brainstem and spinal cord.
|
| Requested From: |
United States |