| Antibody Catalog ID: |
LS-B2564 |
| Antibody Validation: |
This antibody replaces catalog number LS-C501. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: |
Activating Transcription Factor 6 (ATF6) |
| Synonyms: |
ATF6, aft-6, atf6, cyclic-amp-dependent transcription factor atf-6 |
| Host: |
ATF6 antibody was produced in Mouse |
| Antigen Species: |
ATF6 antibody was raised against Human |
| Clonality: |
Monoclonal |
| Isotype: |
IgG1,k |
| Antibody Clone Name: |
70B1413.1 |
| Antigen Type: |
Recombinant protein |
| Immunogen: |
ATF6 antibody was raised against recombinant protein |
| Antibody Specificity: |
A partial protein containing amino acids 1-273 of human ATF6.
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| Antigen Modification: |
aa1-273
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| Predicted Species Reactivity: |
Hamster, Mouse, Rat
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| Purification: |
Protein G Column |
| Presentation: |
PBS, 0.05% BSA, 0.05% sodium azide. |
| Recommended Storage: |
Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: |
Immunohistochemistry: LS-B2564 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2564 was determined to be 10 ug/ml. |
| Antibody Uses: |
Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml ), Immunocytochemistry (ICC), Western blot (WB) (1 - 3 µg/ml ), Chromatin Immunoprecipitation (ChrIP) (Optimal dilution to be determined by the researcher) |
| Size: |
50 µg |
| Concentration: |
0.5 mg/ml |
| Antibody Price (USD): |
$350 |
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Immunohistochemistry Image:
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| Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) |
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Western Blot Image:
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| Western blot analysis of ATF6 in NIH3T3 cell lysate using antibody at 3 ug/ml. A band corresponding to full-length ATF6 was detected. We have not characterized the ~36 kDa observed band; it may be an ATF6 breakdown/cleavage product. |
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Western Blot Image:
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| Western blot analysis of ATF6. Lane 1, 293 cells transfected with full-length ATF6.* Lane 2, 293 cells transfected with partial length ATF6 (amino acids 1-373).* Lane 3, Untransfected 293 cells. Western blots were probed with 4 ug/ml of the ATF6 monoclon |
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Related Activating Transcription Factor 6 (ATF6) Antibodies (5)
|
| Target | Catalog | Type | Form | Epitope | Assay | Reactivity | Size | Price | Buy |
| Activating Transcription Factor 6 (ATF6) Antibody | LS-B2516 | Rabbit | | C-Terminus | | Human | 50 µg | $350 |  |
| Activating Transcription Factor 6 (ATF6) Antibody | LS-B2430 | Rabbit | | N-Terminus | | Human | 50 µg | $350 | |
| Activating Transcription Factor 6 (ATF6) Antibody | LS-B2846 | Rabbit IgG | | N-Terminus | | Human | 50 µg | $385 | |
| Activating Transcription Factor 6 (ATF6) Antibody | LS-C3517 | Rabbit IgG | | C-Terminus | | Human | 100 µg | $450 | |
| Activating Transcription Factor 6 (ATF6) Antibody | LS-C63631 | Rabbit IgG | | aa635-647 | | Human | 100 µl | $510 | |
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Antibody Summary:
ATF6 isa constitutively expressed,endoplasmic reticulum (ER) membrane-anchored transcription factor. ATF6 is a key transcriptional activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER (reviewed in Shen and Prywes, and Groenendyk and Michalak). The C-terminus of ATF6 is located in the ER lumen andits N-terminal DNA binding domain faces the cytosol. AFT6plays key role in the ER stress response by transmitting the ER stress signal across the ER membrane into the nucleus. The induction of new gene expression by ATF6 is an important aspect of the ER stress response. In response to stress, ATF6 translocates from the ER to the Golgi. The 90 kDa full-length ATF6 is processed within theGolgi to its active 50 kDa form through sequential cleavage by site-1 and site-2 proteases (S1P and S2P). Proteolytic activation of ATF6 in the ER stress response is a mechanism to regulate membrane-bound factors,and isreferred to asregulated intramembrane proteolysis. The N-terminal active ATF6translocates to the nucleus where it binds to ER stress-response elements in ER stress-response genes (ERSRGs). ATF6 is a potent transcriptional activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimentaltreatment (Shen and Prywes, 2005).
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| Requested From: |
United States |